ANKRD32 Protein (AA 1-1058) (His tag)

Catalog No. ABIN7555485

Product Details

Target: ANKRD32 (Ankyrin Repeat Domain 32 (ANKRD32))

Protein Type: Recombinant

Protein Characteristics: AA 1-1058

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This ANKRD32 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant SLF1 Protein expressed in mammalian cells.

Sequence: MEDGTPKHII QMTGFKMEEK EALVKLLLKL DCTFIKSEKY KNCTHLIAER LCKSEKFLAA CAAGKWILTK DYIIHSAKSG RWLDETTYEW GYKIEKDSRY SPQMQSAPKR WREELKRTGA PGAFHRWKVV LLVRTDKRSD SLIRVLEAGK ANVILPKSSP SGITHVIASN ARIKAEKEKD NFKAPFYPIQ YLGDFLLEKE IQNDEDSQTN SVWTEHSNEE TNKDFRKDAG FLEMKGALRE TMYRTQKEMQ NHEDVNVGSI LIQHHKKEKF SGSSKDLKFV KMRNTFGSHT YENQKEIKKK DEDIQRSYTL RRKRKKGKES NCKKGVEHEK IKSTLRRHIY NRDQKEMKNS IFAEYAKESK AMAIKTDVDV VEIKNTLRKH IYRAQAVRYN CIRIDKQPVY NVEVKNAEFP RGVLNLIESL IEGHFFKEAI EELSTLQAHY IPPVCVLHAL LENVLQDNID TFSGRYFHIL SALLHLHPPW KSPAMSRYYL ELFQCPTCMK GAWSLVEVLI RSCLFNESFC HQISENIGSK VLHLTLLKFF FNLIESEVQH LSQKLYDWSD SQNLKITGKA MLLEIFWSGS ETSGLLTKPV NMLLEWTIYS HKEKFKSNDV FKHELAYLLA GILGAAIDYW IFLGLKMGRN VMRHMSDDLG SYVSLSCDDF SSQELEIFIC SFSSSWLQMF VAEAVFKKLC LQSSGSVSSE PLSLQKMVYS YLPALGKTGV LGSGKIQVSK KIGQRPCFDS QRTLLMLNGT KQKQVEGLPE LLDLNLAKCS SSLKKLKKKS EGELSCSKEN CPSVVKKMNF HKTNLKGETA LHRACINNQV EKLILLLSLP GIDINVKDNA GWTPLHEACN YGNTVCVQEI LQRCPEVDLL TQVDGVTPLH DALSNGHVEI GKLLLQHGGP VLLQQRNAKG ELPLDYVVSP QIKEELFAIT KIEDTVENFH AQAEKHFHYQ QLEFGSFLLS RMLLNFCSIF DLSSEFILAS KGLTHLNELL MACKSHKETT SVHTDWLLDL YAGNIKTLQK LPHILKELPE NLKVCPGVHT EALMITLEMM CRSVMEFS Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ANKRD32 (Ankyrin Repeat Domain 32 (ANKRD32))

Alternative Name: SLF1 (ANKRD32 Products)

Background: SMC5-SMC6 complex localization factor protein 1 (Ankyrin repeat domain-containing protein 32) (BRCT domain-containing protein 1) (Smc5/6 localization factor 1),FUNCTION: Plays a role in the DNA damage response (DDR) pathway by regulating postreplication repair of UV-damaged DNA and genomic stability maintenance (PubMed:25931565). The SLF1-SLF2 complex acts to link RAD18 with the SMC5-SMC6 complex at replication-coupled interstrand cross-links (ICL) and DNA double-strand breaks (DSBs) sites on chromatin during DNA repair in response to stalled replication forks (PubMed:25931565). Promotes the recruitment of SLF2 and the SMC5-SMC6 complex to DNA lesions (PubMed:25931565, PubMed:36373674). {ECO:0000269|PubMed:25931565, ECO:0000269|PubMed:36373674}.

Molecular Weight: 121.1 kDa

UniProt: Q9BQI6

AlphaFold: Q9BQI6