SIPA1 Protein (AA 1-1042) (His tag)

Catalog No. ABIN7555568

This Recombinant SIPA1 protein is expressed in HEK-293 Cells.

Quick Overview for SIPA1 Protein (AA 1-1042) (His tag) (ABIN7555568)

Target: SIPA1 (Signal-Induced Proliferation-Associated 1 (SIPA1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1042

Purification tag / Conjugate: This SIPA1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant SIPA1 Protein expressed in mammalian cells.

Sequence: MPMWAGGVGS PRRGMAPAST DDLFARKLRQ PARPPLTPHT FEPRPVRGPL LRSGSDAGEA RPPTPASPRA RAHSHEEASR PAATSTRLFT DPLALLGLPA EEPEPAFPPV LEPRWFAHYD VQSLLFDWAP RSQGMGSHSE ASSGTLASAE DQAASSDLLH GAPGFVCELG GEGELGLGGP ASPPVPPALP NAAVSILEEP QNRTSAYSLE HADLGAGYYR KYFYGKEHQN FFGMDESLGP VAVSLRREEK EGSGGGTLHS YRVIVRTTQL RTLRGTISED ALPPGPPRGL SPRKLLEHVA PQLSPSCLRL GSASPKVPRT LLTLDEQVLS FQRKVGILYC RAGQGSEEEM YNNQEAGPAF MQFLTLLGDV VRLKGFESYR AQLDTKTDST GTHSLYTTYQ DHEIMFHVST MLPYTPNNQQ QLLRKRHIGN DIVTIVFQEP GSKPFCPTTI RSHFQHVFLV VRAHTPCTPH TTYRVAVSRT QDTPAFGPAL PAGGGPFAAN ADFRAFLLAK ALNGEQAAGH ARQFHAMATR TRQQYLQDLA TNEVTTTSLD SASRFGLPSL GGRRRAAPRG PGAELQAAGS LVWGVRAAPG ARVAAGAQAS GPEGIEVPCL LGISAEALVL VAPRDGRVVF NCACRDVLAW TFSEQQLDLY HGRGEAITLR FDGSPGQAVG EVVARLQLVS RGCETRELAL PRDGQGRLGF EVDAEGFVTH VERFTFAETA GLRPGARLLR VCGQTLPSLR PEAAAQLLRS APKVCVTVLP PDESGRPRRS FSELYTLSLQ EPSRRGAPDP VQDEVQGVTL LPTTKQLLHL CLQDGGSPPG PGDLAEERTE FLHSQNSLSP RSSLSDEAPV LPNTTPDLLL ATTAKPSVPS ADSETPLTQD RPGSPSGSED KGNPAPELRA SFLPRTLSLR NSISRIMSEA GSGTLEDEWQ AISEIASTCN TILESLSREG QPIPESGDPK GTPKSDAEPE PGNLSEKVSH LESMLRKLQE DLQKEKADRA ALEEEVRSLR HNNRRLQAES ESAATRLLLA SKQLGSPTAD LA Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SIPA1 (Signal-Induced Proliferation-Associated 1 (SIPA1))

Alternative Name: SIPA1

Background: Signal-induced proliferation-associated protein 1 (Sipa-1) (GTPase-activating protein Spa-1) (p130 SPA-1),FUNCTION: GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting them to the putatively inactive GDP-bound state (PubMed:9346962). Affects cell cycle progression (By similarity). {ECO:0000250|UniProtKB:P46062, ECO:0000269|PubMed:9346962}.

Molecular Weight: 112.1 kDa

UniProt: Q96FS4

AlphaFold: Q96FS4

Pathways: Response to Water Deprivation