WHAMM Protein (AA 1-809) (His tag)

Catalog No. ABIN7556003

This Recombinant WHAMM protein is expressed in HEK-293 Cells.

Quick Overview for WHAMM Protein (AA 1-809) (His tag) (ABIN7556003)

Target: WHAMM (WAS Protein Homolog Associated with Actin, Golgi Membranes and Microtubules (WHAMM))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-809

Purification tag / Conjugate: This WHAMM protein is labelled with His tag.

Purpose: Predefined custom protein recombinant WHAMM Protein expressed in mammalian cells.

Sequence: MEDEQPDSLE GWVPVREGLF AEPERHRLRF LVAWNGAEGK FAVTCHDRTA QQRRLREGAR LGPEPEPKPE AAVSPSSWAG LLSAAGLRGA HRQLAALWPP LERCFPRLPP ELDVGGGGAW GLGLGLWALL WPTRAGPGEA ALQELCGQLE RYLGAAADGC GGATVRDALF PAEGGAADCE SPREFRERAL RARWVEADAR LRQVIQGHGK ANTMVALMNV YQEEDEAYQE LVTVATMFFQ YLLQPFRAMR EVATLCKLDI LKSLDEDDLG PRRVVALEKE AEEWTRRAEE AVVSIQDITV NYFKETVKAL AGMQKEMEQD AKRFGQAAWA TAIPRLEKLQ LMLARETLQL MRAKELCLNH KRAEIQGKME DLPEQEKNTN VVDELEIQFY EIQLELYEVK FEILKNEEIL LTTQLDSLKR LIKEKQDEVV YYDPCENPEE LKVIDCVVGL QDDKNLEVKE LRRQCQQLES KRGRICAKRA SLRSRKDQCK ENHRFRLQQA EESIRYSRQH HSIQMKRDKI KEEEQKKKEW INQERQKTLQ RLRSFKDKRL AQSVRNTSGS EPVAPNLPSD LSQQMCLPAS HAVSVIHPSS RKTRGVPLSE AGNVKSPKCQ NCHGNIPVQV FVPVGDQTHS KSSEELSLPP PPPPPPPPPP PPPPPPPPLR ALSSSSQAAT HQNLGFRAPV KDDQPRPLVC ESPAERPRDS LESFSCPGSM DEVLASLRHG RAPLRKVEVP AVRPPHASIN EHILAAIRQG VKLKKVHPDL GPNPSSKPTS NRRTSDLERS IKAALQRIKR VSADSEEDSD EQDPGQWDG Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: WHAMM (WAS Protein Homolog Associated with Actin, Golgi Membranes and Microtubules (WHAMM))

Alternative Name: WHAMM

Background: WASP homolog-associated protein with actin, membranes and microtubules (WAS protein homology region 2 domain-containing protein 1) (WH2 domain-containing protein 1),FUNCTION: Acts as a nucleation-promoting factor (NPF) that stimulates Arp2/3-mediated actin polymerization both at the Golgi apparatus and along tubular membranes. Its activity in membrane tubulation requires F-actin and interaction with microtubules. Proposed to use coordinated actin-nucleating and microtubule-binding activities of distinct WHAMM molecules to drive membrane tubule elongation, when MT-bound can recruit and remodel membrane vesicles but is prevented to activate the Arp2/3 complex. Involved as a regulator of Golgi positioning and morphology. Participates in vesicle transport between the reticulum endoplasmic and the Golgi complex. Required for RhoD-dependent actin reorganization such as in cell adhesion and cell migration. {ECO:0000269|PubMed:18614018, ECO:0000269|PubMed:23027905, ECO:0000269|PubMed:23087206}.

Molecular Weight: 90.9 kDa

UniProt: Q8TF30

AlphaFold: Q8TF30