ZRANB3 Protein (AA 1-1079) (His tag)

Catalog No. ABIN7556124

Recombinant ZRANB3 protein expressed in HEK-293 Cells.

Quick Overview for ZRANB3 Protein (AA 1-1079) (His tag) (ABIN7556124)

Target: ZRANB3 (Zinc Finger, RAN-Binding Domain Containing 3 (ZRANB3))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1079

Purification tag / Conjugate: This ZRANB3 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant ZRANB3 Protein expressed in mammalian cells.

Sequence: MPRVHNIKKS LTPHISCVTN ESDNLLDFLP DRLRAKLLPF QKDGIIFALK RNGRCMVADE MGLGKTIQAI GITYFYKEEW PLLIVVPSSL RYPWTEEIEK WIPELSPEEI NVIQNKTDVR RMSTSKVTVL GYGLLTADAK TLIDALNNQN FKVVIVDESH YMKSRNATRS RILLPIVQKA RRAILLTGTP ALGRPEELFM QIEALFPQKF GRWTDYAKRY CNAHIRYFGK RPQWDCRGAS NLNELHQLLS DIMIRRLKTE VLTQLPPKVR QRIPFDLPSA AAKELNTSFE EWEKIMRTPN SGAMETVMGL ITRMFKQTAI AKAGAVKDYI KMMLQNDSLK FLVFAHHLSM LQACTEAVIE NKTRYIRIDG SVSSSERIHL VNQFQKDPDT RVAILSIQAA GQGLTFTAAS HVVFAELYWD PGHIKQAEDR AHRIGQCSSV NIHYLIANGT LDTLMWGMLN RKAQVTGSTL NGRKEKIQAE EGDKEKWDFL QFAEAWTPND SSEELRKEAL FTHFEKEKQH DIRSFFVPQP KKRQLMTSCD ESKRFREENT VVSSDPTKTA ARDIIDYESD VEPETKRLKL AASEDHCSPS EETPSQSKQI RTPLVESVQE AKAQLTTPAF PVEGWQCSLC TYINNSELPY CEMCETPQGS AVMQIDSLNH IQDKNEKDDS QKDTSKKVQT ISDCEKQALA QSEPGQLADS KEETPKIEKE DGLTSQPGNE QWKSSDTLPV YDTLMFCASR NTDRIHIYTK DGKQMSCNFI PLDIKLDLWE DLPASFQLKQ YRSLILRFVR EWSSLTAMKQ RIIRKSGQLF CSPILALEEI TKQQTKQNCT KRYITKEDVA VASMDKVKNV GGHVRLITKE SRPRDPFTKK LLEDGACVPF LNPYTVQADL TVKPSTSKGY LQAVDNEGNP LCLRCQQPTC QTKQACKANS WDSRFCSLKC QEEFWIRSNN SYLRAKVFET EHGVCQLCNV NAQELFLRLR DAPKSQRKNL LYATWTSKLP LEQLNEMIRN PGEGHFWQVD HIKPVYGGGG QCSLDNLQTL CTVCHKERTA RQAKERSQVR RQSLASKHGS DITRFLVKK Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ZRANB3 (Zinc Finger, RAN-Binding Domain Containing 3 (ZRANB3))

Alternative Name: ZRANB3

Background: DNA annealing helicase and endonuclease ZRANB3 (Annealing helicase 2) (AH2) (Zinc finger Ran-binding domain-containing protein 3) [Includes: DNA annealing helicase ZRANB3 (EC 3.6.4.-), Endonuclease ZRANB3 (EC 3.1.-.-)],FUNCTION: DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events (PubMed:21078962, PubMed:22704558, PubMed:22705370, PubMed:22759634, PubMed:26884333). Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase (PubMed:22759634). In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks (PubMed:22705370, PubMed:22704558). {ECO:0000269|PubMed:21078962, ECO:0000269|PubMed:22704558, ECO:0000269|PubMed:22705370, ECO:0000269|PubMed:22759634, ECO:0000269|PubMed:26884333}.

Molecular Weight: 123.2 kDa

UniProt: Q5FWF4

AlphaFold: Q5FWF4