Synaptotagmin V Protein (SYT5) (AA 1-386) (His tag)
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- Target See all Synaptotagmin V (SYT5) Proteins
- Synaptotagmin V (SYT5)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-386
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Synaptotagmin V protein is labelled with His tag.
- Purpose
- Predefined custom protein recombinant Syt5 Protein expressed in mammalian cells.
- Sequence
- MFPEPPTLGS PAPKTPPDSS RIRQGAVPAW VLATIVLGSG LLVFSSCFCL YRKRCRRRMG KKSQAQAQVH LQEVKELGRS YIDKVQPEIE ELDRSPSMPG QQVSDKHQLG RLQYSLDYDF QTGQLLVGIL QAQGLAALDL GGSSDPYVSV YLLPDKRRRH ETKVHRQTLN PHFGETFAFK VPYVELGGRV LVMAVYDFDR FSRNDAIGEV RVPMSSVNLG RPVQAWRELQ VAPKEEQEKL GDICFSLRYV PTAGKLTVIV LEAKNLKKMD VGGLSDPYVK VHLLQGGKKV RKKKTTIKKN TLNPYYNEAF SFEVPCDQVQ KVQVELTVLD YDKLGKNEAI GRVAVGAAVG GAGLRHWADM LANPRRPIAQ WHSLRPPDRA RPIPAP Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
- Top Product
- Discover our top product SYT5 Protein
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Synaptotagmin V (SYT5) (AA 1-386) protein (His tag)
custom-made SYT5 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Synaptotagmin V (SYT5) (AA 109-386) protein (His tag)SYT5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Synaptotagmin V (SYT5) protein (Myc-DYKDDDDK Tag)SYT5 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- Synaptotagmin V (SYT5)
- Alternative Name
- Syt5 (SYT5 Products)
- Background
- Synaptotagmin-5 (Synaptotagmin IX) (Synaptotagmin V) (SytV),FUNCTION: May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Regulates the Ca(2+)-dependent secretion of norepinephrine in PC12 cells. Required for export from the endocytic recycling compartment to the cell surface. {ECO:0000269|PubMed:11751925}.
- Molecular Weight
- 43.1 kDa
- UniProt
- Q9R0N5
- AlphaFold
- Q9R0N5
- Pathways
- Synaptic Vesicle Exocytosis
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