PREB Protein (AA 1-417) (His tag)

Catalog No. ABIN7556287

This Recombinant PREB protein is produced in HEK-293 Cells.

Quick Overview for PREB Protein (AA 1-417) (His tag) (ABIN7556287)

Target: PREB (Prolactin Regulatory Element Binding (PREB))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-417

Purification tag / Conjugate: This PREB protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Preb Protein expressed in mammalian cells.

Sequence: MGRRRGVELY RAPFPLYALR IDPKTGLLIA AGGGGAAKTG IKNGVHFLQL ELINGCLSAS LLHSHDTETR ATMNLALAGD ILAAGQDAQC QLLRFQVHQQ KGSKAEKSGS KEQGPRQRKG APPAEKKSGA QVHPEGVELK VKNLEAVQTD FSNEPLQKVV CFNHDNTLLA TGGTDGHVRV WKVPSLEKVL EFKAHEGEIG DLTLGPDGKL VTVGWDFKAS VWQKDQLVTQ LQWQENGPAS SNTPYRYQAC RFGQVPDQLG GLRLFTVQIP HKRLRQPPPC YLTAWDSSTF LPLRTRSCGH EVISCLSVSD SGTFLGLGTV TGSVAIYIAF SLQRLYYVKE AHGIVVTDVT FLPEKGCGPK LLGPHETALF SVAVDSRCQL HLLPSRRSVP VWLLLLLCVG LIIVTILLLQ TAFPGFL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: PREB (Prolactin Regulatory Element Binding (PREB))

Alternative Name: Preb

Background: Prolactin regulatory element-binding protein (Mammalian guanine nucleotide exchange factor mSec12),FUNCTION: Guanine nucleotide exchange factor that specifically activates the small GTPase SAR1B. Mediates the recruitment of SAR1B and other COPII coat components to endoplasmic reticulum membranes and is therefore required for the formation of COPII transport vesicles from the ER. {ECO:0000269|PubMed:11422940}., FUNCTION: Was first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription. {ECO:0000250|UniProtKB:Q9WTV0}.

Molecular Weight: 45.4 kDa

UniProt: Q9WUQ2

AlphaFold: Q9WUQ2

Pathways: ER-Nucleus Signaling