NME3 Protein (AA 1-169) (His tag)

Catalog No. ABIN7556307

Product Details

Target: NME3 (NME/NM23 Nucleoside Diphosphate Kinase 3 (NME3))

Protein Type: Recombinant

Protein Characteristics: AA 1-169

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This NME3 protein is labelled with His tag.

Purpose: Made-to-order recombinant Nme3 Protein expressed in mammalian cells.

Sequence: MICLVLTIFA NLFPSAYSGV NERTFLAVKP DGVQRRLVGE IVRRFERKGF KLVALKLVQA SEELLREHYV ELREKPFYSR LVKYMSSGPV VAMVWQGLDV VHASRALIGA TDPGDAMPGT IRGDFCMEVG KNVIHGSDSV ESAHREIALW FREAELLCWE DSAGHWLYE Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: NME3 (NME/NM23 Nucleoside Diphosphate Kinase 3 (NME3))

Alternative Name: Nme3 (NME3 Products)

Background: Nucleoside diphosphate kinase 3 (NDK 3) (NDP kinase 3) (EC 2.7.4.6) (DR-nm23) (Nucleoside diphosphate kinase C) (NDPKC) (nm23-M3),FUNCTION: Catalyzes the phosphorylation of ribonucleosides and deoxyribonucleoside diphosphates, other than ATP, into the corresponding triphosphates with ATP as the major phosphate donor. The ATP gamma phosphate is transferred to the nucleoside diphosphate beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Through the catalyzed exchange of gamma-phosphate between di- and triphosphonucleosides participates in regulation of intracellular nucleotide homeostasis (PubMed:22438820). Inhibits granulocyte differentiation (By similarity). May be required for ciliary function during renal development (By similarity). {ECO:0000250|UniProtKB:Q13232, ECO:0000250|UniProtKB:Q9PTF3, ECO:0000269|PubMed:22438820}., FUNCTION: Independently of its kinase activity, facilitates mitochondrial tethering prior to membrane fusion through its direct membrane-binding and hexamerization. Implicated in repair of both single- and double-stranded breaks in DNA through its association with the ribonucleotide reductase complex (RNR complex) via its interaction with the histone acetyltransferase KAT5, this interaction enables recruitment of NME3 at DNA damage sites where it plays a role in the repair of DNA, independently of its kinase activity. {ECO:0000250|UniProtKB:Q13232}.

Molecular Weight: 19.1 kDa

UniProt: Q9WV85

AlphaFold: Q9WV85

Pathways: Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process