Claudin 18 Protein (CLDN18) (AA 1-264) (His tag)

Catalog No. ABIN7556497

This Recombinant Claudin 18 protein is produced in HEK-293 Cells.

Quick Overview for Claudin 18 Protein (CLDN18) (AA 1-264) (His tag) (ABIN7556497)

Target: Claudin 18 (CLDN18)

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-264

Purification tag / Conjugate: This Claudin 18 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Cldn18 Protein expressed in mammalian cells.

Sequence: MATTTCQVVG LLLSLLGLAG CIAATGMDMW STQDLYDNPV TAVFQYEGLW RSCVQQSSGF TECRPYFTIL GLPAMLQAVR ALMIVGIVLG VIGILVSIFA LKCIRIGSMD DSAKAKMTLT SGILFIISGI CAIIGVSVFA NMLVTNFWMS TANMYSGMGG MGGMVQTVQT RYTFGAALFV GWVAGGLTLI GGVMMCIACR GLTPDDSNFK AVSYHASGQN VAYRPGGFKA STGFGSNTRN KKIYDGGART EDDEQSHPTK YDYV Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Claudin 18 (CLDN18)

Alternative Name: Cldn18

Background: Claudin-18,FUNCTION: Involved in alveolar fluid homeostasis via regulation of alveolar epithelial tight junction composition and therefore ion transport and solute permeability, potentially via downstream regulation of the actin cytoskeleton organization and beta-2-adrenergic signaling (PubMed:24588076). Required for lung alveolarization and maintenance of the paracellular alveolar epithelial barrier (PubMed:24787463). Acts to maintain epithelial progenitor cell proliferation and organ size, via regulation of YAP1 localization away from the nucleus and thereby restriction of YAP1 target gene transcription (PubMed:29400695). Acts as a negative regulator of RANKL-induced osteoclast differentiation, potentially via relocation of TJP2/ZO-2 away from the nucleus, subsequently involved in bone resorption in response to calcium deficiency (PubMed:22437732). Mediates the osteoprotective effects of estrogen, potentially via acting downstream of estrogen signaling independently of RANKL signaling pathways (PubMed:23299504). {ECO:0000269|PubMed:22437732, ECO:0000269|PubMed:23299504, ECO:0000269|PubMed:24588076, ECO:0000269|PubMed:24787463, ECO:0000269|PubMed:29400695}., FUNCTION: [Isoform A1.1]: Involved in the maintenance of homeostasis of the alveolar microenvironment via regulation of  pH and subsequent T-cell activation in the alveolar space, is therefore indirectly involved in limiting C. neoformans infection. {ECO:0000269|PubMed:34702961}., FUNCTION: [Isoform A2.1]: Required for the formation of the gastric paracellular barrier via its role in tight junction formation, thereby involved in the response to gastric acidification. {ECO:0000269|PubMed:22079592}.

Molecular Weight: 28.1 kDa

UniProt: P56857

AlphaFold: P56857

Pathways: Cell-Cell Junction Organization, Hepatitis C