ANP32E Protein (AA 1-260) (His tag)

Catalog No. ABIN7556831

This Recombinant ANP32E protein is expressed in HEK-293 Cells.

Quick Overview for ANP32E Protein (AA 1-260) (His tag) (ABIN7556831)

Target: ANP32E (Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member E (ANP32E))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-260

Purification tag / Conjugate: This ANP32E protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Anp32e Protein expressed in mammalian cells.

Sequence: MEMKKKINME LKNRAPEEVT ELVLDNCLCV NGEIEGLNDT FKELEFLSMA NVELSSLARL PSLNKLRKLE LSDNIISGGL EVLAEKCPNL TYLNLSGNKI KDLSTVEALQ NLKNLKSLDL FNCEITNLED YRESIFELLQ QITYLDGFDQ EDNEAPDSEE EDDDDEDGDE DEEDEDEDEA GPPEGYEEEE DDDEDEAGSE VGEGEEEVGL SYLMKDEIQD EEDDDDYVDE GEEEEEEEEE GLRGEKRKRD AEDDGEEDDD Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ANP32E (Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member E (ANP32E))

Alternative Name: Anp32e

Background: Acidic leucine-rich nuclear phosphoprotein 32 family member E (Cerebellar postnatal development protein 1) (LANP-like protein) (LANP-L),FUNCTION: Histone chaperone that specifically mediates the genome-wide removal of histone H2A.Z/H2AZ1 from the nucleosome: removes H2A.Z/H2AZ1 from its normal sites of deposition, especially from enhancer and insulator regions. Not involved in deposition of H2A.Z/H2AZ1 in the nucleosome. May stabilize the evicted H2A.Z/H2AZ1-H2B dimer, thus shifting the equilibrium towards dissociation and the off-chromatin state (PubMed:24463511). Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis. {ECO:0000269|PubMed:11430900, ECO:0000269|PubMed:14964690, ECO:0000269|PubMed:16420440, ECO:0000269|PubMed:24463511}.

Molecular Weight: 29.6 kDa

UniProt: P97822

AlphaFold: P97822