Nebulette Protein (NEBL) (AA 1-452) (His tag)

Catalog No. ABIN7556937

Recombinant Nebulette protein expressed in HEK-293 Cells.

Quick Overview for Nebulette Protein (NEBL) (AA 1-452) (His tag) (ABIN7556937)

Target: Nebulette (NEBL)

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-452

Purification tag / Conjugate: This Nebulette protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Nebl Protein expressed in mammalian cells.

Sequence: MKVPVSGDVK EETEEENVEQ EENQEAKVSL KPVIEDLSME LARKCTELIS DIHYKEEYKK SKDKCTFVTD TPMLNHVKNI GAFISEAKYK GTIKADLSNC LYKDMPATID SVFAREVSQL QSEVAYKQKH EAEKGFSDYT HMKEPPEVRR AMEVNRHQSN ISYRKDMQGT HTYTAELDRP DIKKATQISK IISDAEYKKG QGIVNKEPSV IGRPDFEHAV GASKLSSQVK YKEKFDNEMK EKSHHYNPLG SAFFRQHQFA AVLASDWEYK RDFEENKGLY HFDAEAPEHL HHKGNATLQS QVKYREEYEK NKGKSMLEFV ETPSYQSSKE AQKMQSEKVY KEDFEKEIKG RSSLDLDKTP AFLHVKHITN LMREKEYKKD LENEIKGKGM ELSSEVLDIQ RAKRASEMAS EKDYKRDLET EIKGKGMQVS TDTLDVQRAK RASEMASQVR MV Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Nebulette (NEBL)

Alternative Name: Nebl

Background: Nebulette (Actin-binding Z-disk protein),FUNCTION: Binds to actin and plays an important role in the assembly of the Z-disk. May functionally link sarcomeric actin to the desmin intermediate filaments in the heart muscle sarcomeres. Isoform 2 might play a role in the assembly of focal adhesion (By similarity). {ECO:0000250|UniProtKB:O76041}.

Molecular Weight: 52.2 kDa

UniProt: Q0II04

AlphaFold: Q0II04