ST3GAL2 Protein (AA 1-350) (His tag)

Catalog No. ABIN7556965

Product Details

Target: ST3GAL2 (ST3 beta-Galactoside alpha-2,3-Sialyltransferase 2 (ST3GAL2))

Protein Type: Recombinant

Protein Characteristics: AA 1-350

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This ST3GAL2 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant St3gal2 Protein expressed in mammalian cells.

Sequence: MKCSLRVWFL SMAFLLVFIM SLLFTYSHHS MATLPYLDSG ALGGTHRVKL VPGYSGLQRL GKEGLLGRNC ACSRCMGDAS TSEWFDSHFD GNISPVWTRD NMNLPPDVQR WWMMLQPQFK SHNTNEVLEK LFQIVPGENP YRFRDPQQCR RCAVVGNSGN LRGSGYGQEV DSHNFIMRMN QAPTVGFEKD VGSRTTHHFM YPESAKNLPA NVSFVLVPFK ALDLMWIASA LSTGQIRFTY APVKSFLRVD KEKVQIYNPA FFKYIHDRWT EHHGRYPSTG MLVLFFALHV CDEVNVYGFG ADSRGNWHHY WENNRYAGEF RKTGVHDADF EAHIIDMLAK ASKIEVYRGN Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ST3GAL2 (ST3 beta-Galactoside alpha-2,3-Sialyltransferase 2 (ST3GAL2))

Alternative Name: St3gal2 (ST3GAL2 Products)

Background: CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2 (Alpha 2,3-ST 2) (Beta-galactoside alpha-2,3-sialyltransferase 2) (EC 2.4.3.4) (Gal-NAc6S) (Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase) (Monosialoganglioside sialyltransferase) (EC 2.4.3.2) (ST3Gal II) (ST3GalII) (ST3GalA.2) (Sialyltransferase 4B) (SIAT4-B),FUNCTION: A beta-galactoside alpha2-3 sialyltransferase primarily involved in terminal sialylation of ganglio and globo series glycolipids (PubMed:8144500, PubMed:9184827). Catalyzes the transfer of sialic acid (N-acetyl-neuraminic acid, Neu5Ac) from the nucleotide sugar donor CMP-Neu5Ac onto acceptor Galbeta-(1->3)-GalNAc-terminated glycoconjugates through an alpha2-3 linkage. Sialylates GM1/GM1a, GA1/asialo-GM1 and GD1b gangliosides to form GD1a, GM1b and GT1b, respectively (PubMed:8144500, PubMed:9184827). Together with ST3GAL3, primarily responsible for biosynthesis of brain GD1a and GT1b that function as ligands for myelin-associated glycoprotein MAG on axons, regulating MAG expression and axonal myelin stability and regeneration (PubMed:22735313). Via GT1b regulates TLR2 signaling in spinal cord microglia in response to nerve injury (PubMed:32030804). Responsible for the sialylation of the pluripotent stem cell- and cancer stem cell-associated antigen SSEA3, forming SSEA4 (By similarity). Sialylates with low efficiency asialofetuin, presumably onto O-glycosidically linked Galbeta-(1->3)-GalNAc-O-Ser (PubMed:8144500). {ECO:0000250|UniProtKB:Q16842, ECO:0000269|PubMed:22735313, ECO:0000269|PubMed:32030804, ECO:0000269|PubMed:8144500, ECO:0000269|PubMed:9184827}.

Molecular Weight: 40.1 kDa

UniProt: Q11204

AlphaFold: Q11204

Pathways: Glycosaminoglycan Metabolic Process