MIDN Protein (AA 1-465) (His tag)
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- Target See all MIDN Proteins
- MIDN (Midnolin (MIDN))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-465
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This MIDN protein is labelled with His tag.
- Purpose
- Predefined custom protein recombinant Midn Protein expressed in mammalian cells.
- Sequence
- MEPQPGGARS CRRGAPGGAC ELNTATESAA PMSLAIHSTT GTRYDLSVPH DETVEGLRKR LSQRLKVPKE RLALLHKDTR LSSGKLQEFG VGDGSKLTLV PTVEAGLMSQ ASRPEQSVMQ ALESLTETQV SDFLSGRSPL TLALRVGDHM MFVQLQLAAQ HAPLQHRHVL AAAAAAAAAA RGDSSVATPV SSPCRPVSSA ARVPPVSSSP SSPVSPSPVT AGSFRSHAAS TTCPEQMDCS PPASSSSTST PGSSPTPRSR KPGAVIESFV NHAPGVFSGT FSGTLHPNCQ DSSGRPRRDI GTILQILNDL LSATRHYQGM PPSLTQLRCH AQCSPASPAP DLTPKTTSCE KLAATSSTSL LQGQSQIRMC KPPGDRLRQT ENRATRCKVE RLQLLLQQKR LRRKARRDAR GPYHWTPSRK AGRSDSSSSG GGGGPSEATG LGLDFEDSVW KPEVNPDIQS EFVVA Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
- Top Product
- Discover our top product MIDN Protein
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Midnolin (MIDN) (AA 1-468) protein (His tag)
custom-made MIDN Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Midnolin (MIDN) (AA 1-468) protein (GST tag)MIDN Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- MIDN (Midnolin (MIDN))
- Alternative Name
- Midn (MIDN Products)
- Background
- Midnolin (Midbrain nucleolar protein),FUNCTION: Facilitates the ubiquitin-independent proteasomal degradation of stimulus-induced transcription factors such as FOSB, EGR1, NR4A1, and IRF4 to the proteasome for degradation (By similarity). Promotes also the degradation of other substrates such as CBX4 (PubMed:27326929). Plays a role in inhibiting the activity of glucokinase GCK and both glucose-induced and basal insulin secretion (By similarity). {ECO:0000250|UniProtKB:D4AE48, ECO:0000250|UniProtKB:Q504T8, ECO:0000269|PubMed:27326929}.
- Molecular Weight
- 49.2 kDa
- UniProt
- Q3TPJ7
- AlphaFold
- Q3TPJ7
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