RNF10 Protein (AA 1-804) (His tag)

Catalog No. ABIN7557205

Product Details

Target: RNF10 (Ring Finger Protein 10 (RNF10))

Protein Type: Recombinant

Protein Characteristics: AA 1-804

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This RNF10 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Rnf10 Protein expressed in mammalian cells.

Sequence: MPQSSPSAAA TASDMDKNSG SNSSSASSGS SKGQQPPRSA SAGPAGESKP KSDGKNSNGS KRYNRKREPS YPKNENFSNQ SRRSNSQKSK TFNKMPPQRG GGSSKPFSSS SNGGRRDEVA EAQRAEFSPA QFSGPKKINL NHLLNFTFEP RGQAGHFEGS GHGGWGKRNK WGHKPFNKEL FLQANCQFVV SEDQDYAAHF ADPDTLVNWD FVEQVRICSH EVPSCPICLY PPTAAKITRC GHIFCWACIL HYLSLSEKTW SKCPICYSSV HKKDLKSVVA TESRQYAVGD TITMQLMKRE KGVLVALPKS KWVNVDHPIN LGDEQLSQYS KLLLASKEQV LHRVVLEEKG ALEQQLAEEK HTPESCFIEA AIQEVKIREE ALSGVAGGGG EVTGVVAALE HLVLMAPLAT ESAFQPRKGV LEYLSAFDDE AAQVCSLDPP GPLALPLVEE EEAVSEPEAC EDAEVADDSL GEGTVGPEMS QEEPITKPGF TQLSSSPCYY FYQAEDGQHM FLHPVNVRCL VREYGSLEQS PEKISATVVE IAGYSMSEDV RQRHRYLSHL PLTCEFSICE LALQPPVVSK ETLEMFSDDI EKRKRQRQKK AREERRRERR IEMEENKRQG RYPEVHIPLE NLQQFPAFNS YTCPSDSALG PTSTEGHGAL SLSPLSRSPG SHADFLLTPL SPTASQGSPS FCVGSLEDDS PFLSFAQMLR VGKAKADGWP KTAPKKDDNS LVPPAPVDSD GESDNSDRVP VPSFQNSFSQ AIEAAFMKLD TPATSDPLSE DRGGKKRKRQ KQKLLFSTSV VHTK Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: RNF10 (Ring Finger Protein 10 (RNF10))

Alternative Name: Rnf10 (RNF10 Products)

Background: E3 ubiquitin-protein ligase RNF10 (EC 2.3.2.27) (RING finger protein 10) (Sid 2705),FUNCTION: E3 ubiquitin-protein ligase that catalyzes monoubiquitination of 40S ribosomal proteins RPS2/us5 and RPS3/us3 in response to ribosome stalling. Part of a ribosome quality control that takes place when ribosomes have stalled during translation initiation (iRQC): RNF10 acts by mediating monoubiquitination of RPS2/us5 and RPS3/us3, promoting their degradation by the proteasome. Also promotes ubiquitination of 40S ribosomal proteins in response to ribosome stalling during translation elongation. The action of RNF10 in iRQC is counteracted by USP10 (By similarity). May also act as a transcriptional factor involved in the regulation of MAG (Myelin-associated glycoprotein) expression. Acts as a regulator of Schwann cell differentiation and myelination (By similarity). {ECO:0000250|UniProtKB:Q5XI59, ECO:0000250|UniProtKB:Q8N5U6}.

Molecular Weight: 88.3 kDa

UniProt: Q3UIW5

AlphaFold: Q3UIW5