GCNT3 Protein (AA 1-437) (His tag)

Catalog No. ABIN7557468

Recombinant GCNT3 protein expressed in HEK-293 Cells.

Quick Overview for GCNT3 Protein (AA 1-437) (His tag) (ABIN7557468)

Target: GCNT3 (Glucosaminyl (N-Acetyl) Transferase 3, Mucin Type (GCNT3))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-437

Purification tag / Conjugate: This GCNT3 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Gcnt3 Protein expressed in mammalian cells.

Sequence: MTSWQRLCWH YRLWTLGCYM LLAILALKLS LRLKCDFDAM DLDSEEFQSQ YCRDLLYKTL KLPAKSSINC SGVIRGEQKA VTQALLNNLE IKKKQQLFTE ADYLRMTADC EHFKTKRKFI QVPLSKEEAS FPIAYSMVVH EKIENFERLL RAVYTPQNVY CVHMDQKSSE PFKQAVRAIV SCFPNVFIAS KLVSVVYASW SRVQADLNCM EDLLQSPVPW KYLLNTCGTD FPIKTNAEMV KALKLLKGQN SMESEVPPPH KKSRWKYHYE VTDTLHMTSK RKTPPPNNLT MFTGNAYMVA SRDFIEHVFS NSKARQLIEW VKDTYSPDEH LWATLQRASW MPGSDPLHRK FDLSDMRAIA RLTKWYDHEG DIENGAPYTS CSGIHQRAVC VYGSGDLHWI LQNHHLLANK FDPKVDDNVL QCLEEYLRHK AIYGTEL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: GCNT3 (Glucosaminyl (N-Acetyl) Transferase 3, Mucin Type (GCNT3))

Alternative Name: Gcnt3

Background: Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 (EC 2.4.1.102) (EC 2.4.1.148) (EC 2.4.1.150) (C2GnT-mucin type) (C2GnT-M) (Mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase),FUNCTION: Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development. {ECO:0000250|UniProtKB:O95395}.

Molecular Weight: 50.7 kDa

UniProt: Q5JCT0

AlphaFold: Q5JCT0

Pathways: Production of Molecular Mediator of Immune Response