TOX Protein (AA 1-526) (His tag)

Catalog No. ABIN7557864

Product Details

Target: TOX (Thymus High Mobility Group Box Protein (TOX))

Protein Type: Recombinant

Protein Characteristics: AA 1-526

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This TOX protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Tox Protein expressed in mammalian cells.

Sequence: MDVRFYPPPA QPAAAPAAPC LGPSPCLDPY YCNKFDGENM YMSMTEPSQD YVPASQSYPG PSLESEDFNI PPITPPSLPD HSLVHLNEVE SGYHSLCHPM NHNGLLPFHP QTMDLPEITV SNMLGQDGAL LSNSISVMQE IGNAEGAQYS SHPQMAAMRP RGQPTDIRQQ ASMMQPGQLT TINQSQLSAQ LGLNMGGTNV AHNSPSPPGS KSATPSPSSS VHEDECEDAS KINGGEKRPA SDMGKKPKTP KKKKKKDPNE PQKPVSAYAL FFRDTQAAIK GQNPNATFGE VSKIVASMWD GLGEEQKQVY KKKTEAAKKE YLKQLAAYRA SLVSKSYTDP VDVKTSQPPQ LVNSKPSVFH GPSQAHSALY LSSHYHQQPG MTPQLTAMHP SLPRNIAPKP NNQMPVTVSI ANMAVSPPPP LQISPPLHQH LSMQQHQSLA MQQPLGSQLP MQVQTALHSP TMQQGFTLQP DYQTIINPTS TAAQVVTQAM EYVRSGCRNP PPQPVDWSTD YCSSGGMQRD KALYLT Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TOX (Thymus High Mobility Group Box Protein (TOX))

Alternative Name: Tox (TOX Products)

Background: Thymocyte selection-associated high mobility group box protein TOX (Thymus high mobility group box protein TOX),FUNCTION: Transcriptional regulator with a major role in neural stem cell commitment and corticogenesis as well as in lymphoid cell development and lymphoid tissue organogenesis (PubMed:25527292, PubMed:20818394, PubMed:11850626, PubMed:18195075, PubMed:15078895, PubMed:25915732). Binds to GC-rich DNA sequences in the proximity of transcription start sites and may alter chromatin structure, modifying access of transcription factors to DNA (PubMed:25527292, PubMed:31207603, PubMed:31207604). During cortical development, controls the neural stem cell pool by inhibiting the switch from proliferative to differentiating progenitors. Beyond progenitor cells, promotes neurite outgrowth in newborn neurons migrating to reach the cortical plate. May activate or repress critical genes for neural stem cell fate such as SOX2, EOMES and ROBO2 (PubMed:25527292). Plays an essential role in the development of lymphoid tissue-inducer (LTi) cells, a subset necessary for the formation of secondary lymphoid organs: peripheral lymph nodes and Peyer's patches (PubMed:20818394). Acts as a developmental checkpoint and regulates thymocyte positive selection toward T cell lineage commitment (PubMed:11850626, PubMed:18195075). Required for the development of various T cell subsets, including CD4-positive helper T cells, CD8-positive cytotoxic T cells, regulatory T cells and CD1D-dependent natural killer T (NKT) cells (PubMed:18195075, PubMed:15078895). Required for the differentiation of common lymphoid progenitors (CMP) to innate lymphoid cells (ILC). May regulate the NOTCH-mediated gene program, promoting differentiation of the ILC lineage (PubMed:25915732). Required at the progenitor phase of NK cell development in the bone marrow to specify NK cell lineage commitment (PubMed:20818394). Upon chronic antigen stimulation, diverts T cell development by promoting the generation of exhaustive T cells, while suppressing effector and memory T cell programming. May regulate the expression of genes encoding inhibitory receptors such as PDCD1 and induce the exhaustion program, to prevent the overstimulation of T cells and activation-induced cell death (PubMed:31207603, PubMed:31207604). {ECO:0000269|PubMed:11850626, ECO:0000269|PubMed:15078895, ECO:0000269|PubMed:18195075, ECO:0000269|PubMed:20818394, ECO:0000269|PubMed:25527292, ECO:0000269|PubMed:25915732, ECO:0000269|PubMed:31207603, ECO:0000269|PubMed:31207604}.

Molecular Weight: 57.2 kDa

UniProt: Q66JW3

AlphaFold: Q66JW3