ISY1 Protein (AA 1-285) (His tag)

Catalog No. ABIN7557890

This Recombinant ISY1 protein is expressed in HEK-293 Cells.

Quick Overview for ISY1 Protein (AA 1-285) (His tag) (ABIN7557890)

Target: ISY1 (ISY1 Splicing Factor Homolog (ISY1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-285

Purification tag / Conjugate: This ISY1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Isy1 Protein expressed in mammalian cells.

Sequence: MARNAEKAMT ALARFRQAQL EEGKVKERRP FLASECTELP KAEKWRRQII GEISKKVAQI QNAGLGEFRI RDLNDEINKL LREKGHWEVR IKELGGPDYG KVGPKMLDHE GKEVPGNRGY KYFGAAKDLP GVRELFEKEP LPPPRKTRAE LMKAIDFEYY GYLDEDDGVI VPLEQEYEKK LRAELVEKWK AEREARLARG EKEEEEEEEE EINIYAVTEE ESDEEGNQEK AGEDGQQKFI AHVPVPSQQE IEEALVRRKK MELLQKYASE TLQAQSEEAK RLLGY Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ISY1 (ISY1 Splicing Factor Homolog (ISY1))

Alternative Name: Isy1

Background: Pre-mRNA-splicing factor ISY1 homolog,FUNCTION: Component of the spliceosome C complex required for the selective processing of microRNAs (miRNAs) during embryonic stem cell differentiation (PubMed:26255770, PubMed:29804889). Required for the biogenesis of all miRNAs from the pri-miR-17-92 primary transcript except miR-92a (PubMed:26255770). Only required for the biogenesis of miR-290 and miR-96 from the pri-miR-290-295 and pri-miR-96-183 primary transcripts, respectively (PubMed:29804889). Required during the transition of embryonic stem cells (ESCs) from the naive to primed state (PubMed:29804889). By enhancing miRNA biogenesis, promotes exit of ESCs from the naive state to an intermediate state of poised pluripotency, which precedes the transition to the primed state (PubMed:29804889). Involved in pre-mRNA splicing as component of the spliceosome. {ECO:0000250|UniProtKB:Q9ULR0, ECO:0000269|PubMed:26255770, ECO:0000269|PubMed:29804889}.

Molecular Weight: 33.0 kDa

UniProt: Q69ZQ2

AlphaFold: Q69ZQ2