DDX31 Protein (AA 1-687) (His tag)
custom-made
DDX31
Origin: Mouse
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all DDX31 products
- DDX31 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 31 (DDX31))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-687
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This DDX31 protein is labelled with His tag.
- Purpose
- Predefined custom protein recombinant Ddx31 Protein expressed in mammalian cells.
- Sequence
- MKRQAEATKR KHQVSSDAPP AKRRSEISSV LAKKASDKET QRTFKGSTHK TFPPKKYLDS IGNGRQEEKP CIKTSSLFKN NPEIPELHST VVKQAREQVF SPEAFQELDL HPHLISTINT VLKMSSMTSV QKQSIPVLLE GRDALVRSQT GSGKTLAYCV PVVQSLQALT SKIQRSDGPY ALVLVPTREL ALQSFDTVQK LLKPFTWIVP GVLMGGEKRK SEKARLRKGI NILISTPGRL VDHIKSTKNL HFNRIRWLIV DEADRILDLG FEKDITVILN AVNAECQKRQ NVLLSATLTE GVTRLVDISL HNPVSISVLD KNCNQPNPKE VASIQLNSFA IPESLDQHVV LVPSKLRLVC LAAFILQKCK FEKNQKMIVF FSSCELVEFH YSLFLHTLLC HSGTPTSEHL PSASWPLKFL RLHGNMEQEE RTSVFHEFSH SETGVLLCTD VASRGLDLPQ VTWIVQYSAP SSPAEYIHRI GRTARIGCHG SSLLILAPSE AEYVNSLASH KINVGEIKME DILAVLAKDD CFKRRQRGAQ KSRASGPQEI RERATVLQTV FEDYVHSSQR MVSWAKKALQ SFIRAYATYP KELKSIFHVR ALHLGHVAKS FGLRDAPRNL SVSAVKKASL KRPHPRRKTQ RKQHLVPAEV LHSEHSSGLE GGATKCRKQG KQQGLQVAPS KPGPWRE Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 31 (DDX31) (AA 1-851) protein (His tag)
custom-made DDX31 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- DDX31 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 31 (DDX31))
- Alternative Name
- Ddx31 (DDX31 Products)
- Background
- Probable ATP-dependent RNA helicase DDX31 (EC 3.6.4.13) (DEAD box protein 31),FUNCTION: Probable ATP-dependent RNA helicase (By similarity). Plays a role in ribosome biogenesis and TP53/p53 regulation through its interaction with NPM1 (By similarity). {ECO:0000250|UniProtKB:Q9H8H2}.
- Molecular Weight
- 76.9 kDa
- UniProt
- Q6NZQ2
- AlphaFold
- Q6NZQ2
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