OPN5 Protein (AA 1-377) (His tag)

Catalog No. ABIN7558122

Recombinant OPN5 protein expressed in HEK-293 Cells.

Quick Overview for OPN5 Protein (AA 1-377) (His tag) (ABIN7558122)

Target: OPN5 (Opsin 5 (OPN5))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-377

Purification tag / Conjugate: This OPN5 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Opn5 Protein expressed in mammalian cells.

Sequence: MALNHTALPQ DERLPHYLRD EDPFASKLSW EADLVAGFYL TIIGILSTFG NGYVLYMSSR RKKKLRPAEI MTINLAVCDL GISVVGKPFT IISCFCHRWV FGWFGCRWYG WAGFFFGCGS LITMTAVSLD RYLKICYLSY GVWLKRKHAY ICLAVIWAYA SFWTTMPLVG LGDYAPEPFG TSCTLDWWLA QASGGGQVFI LSILFFCLLL PTAVIVFSYA KIIAKVKSSS KEVAHFDSRI HSSHVLEVKL TKVAMLICAG FLIAWIPYAV VSVWSAFGRP DSIPIQLSVV PTLLAKSAAM YNPIIYQVID YRFACCQAGG LRGTKKKSLE DFRLHTVTAV RKSSAVLEIH PESSSRFTSA HVMDGESHSN DGDCGKK Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: OPN5 (Opsin 5 (OPN5))

Alternative Name: Opn5

Background: Opsin-5 (G-protein coupled receptor 136) (G-protein coupled receptor PGR12) (Neuropsin),FUNCTION: G-protein coupled receptor which selectively activates G(i) type G proteins via ultraviolet A (UVA) light-mediated activation in the retina (PubMed:22043319). Preferentially binds the chromophore 11-cis retinal and is a bistable protein that displays emission peaks at 380 nm (UVA light) and 470 nm (blue light) (PubMed:22043319, PubMed:31607531). Required for the light-response in the inner plexiform layer, and contributes to the regulation of the light-response in the nerve fiber layer, via phosphorylated DAT/SLC6A3 dopamine uptake (PubMed:30936473). Involved in local corneal and retinal circadian rhythm photoentrainment via modulation of the UVA light-induced phase-shift of the retina clock (PubMed:26392540, PubMed:30240620). Acts as a circadian photoreceptor in the outer ear and vibrissal pads, via modulation of circadian clock-gene expression in response to violet light during the light-to-dark transition phase and night phase of the circadian cycle (PubMed:31607531). Required in the retina to negatively regulate hyaloid vessel regression during postnatal development via light-dependent OPN5-SLC32A1-DRD2-VEGFR2 signaling (PubMed:30936473). Involved in the light-dependent regulation of retina and vitreous compartment dopamine levels (PubMed:30936473). {ECO:0000269|PubMed:22043319, ECO:0000269|PubMed:26392540, ECO:0000269|PubMed:30240620, ECO:0000269|PubMed:30936473, ECO:0000269|PubMed:31607531}.

Molecular Weight: 42.0 kDa

UniProt: Q6VZZ7

AlphaFold: Q6VZZ7