FBLIM1 Protein (AA 1-375) (His tag)

Catalog No. ABIN7558155

Product Details

Target: FBLIM1 (Filamin Binding LIM Protein 1 (FBLIM1))

Protein Type: Recombinant

Protein Characteristics: AA 1-375

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This FBLIM1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Fblim1 Protein expressed in mammalian cells.

Sequence: MASKPEKRVA SSVFITLAPP RRDVAVSEEV GQAACEARRA RPWEMLPTKT PGAAVGRSPK TWTPSGKTNA SLSGVTPQLS NGGCSLPPPS LNEEDLDLPP PPPPPSAYLP LPEEEPPVLP GKSLISDLEQ LHLPPPPPPP PPQAPSKGSS VHPPPGHAIP SEEELPPPPE EPVTLPEREV STDVCGFCHK PVSPRELAVE AMKRQYHAQC FTCRTCRRQL AGQRFYQKDG RPLCEPCYQD TLEKCGKCGE VVQEHVIRAL GKAFHPPCFT CVTCARCISD ESFALDSQNQ VYCVADFYRK FAPVCSICEN PIIPRDGKDA FKIECMGRNF HENCYRCEDC SVLLSVEPTD QGCYPLNDHL FCKPCHLKRS AAGCC Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: FBLIM1 (Filamin Binding LIM Protein 1 (FBLIM1))

Alternative Name: Fblim1 (FBLIM1 Products)

Background: Filamin-binding LIM protein 1 (FBLP-1) (CSX-associated LIM),FUNCTION: Serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. Is implicated in cell shape modulation (spreading) and motility. May participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. May also regulate the assembly of filamin-containing signaling complexes that control actin assembly. Promotes dissociation of FLNA from ITGB3 and ITGB7. Promotes activation of integrins and regulates integrin-mediated cell-cell adhesion (By similarity). {ECO:0000250}.

Molecular Weight: 41.0 kDa

UniProt: Q71FD7

AlphaFold: Q71FD7