TRIM17 / RNF16 Protein (TRIM17) (AA 1-477) (His tag)

Catalog No. ABIN7558246

Product Details

Target: TRIM17 / RNF16 (TRIM17)

Protein Type: Recombinant

Protein Characteristics: AA 1-477

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This TRIM17 / RNF16 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Trim17 Protein expressed in mammalian cells.

Sequence: MDAVELARRL QEEATCSICL DYFTDPVMTA CGHNFCRECI QMSWEKGKVK KGKKKQKGSF PCPECREMSP QRNLRPNRLL TKVAEMARQH PGLQKRDLCQ AHQEPLKLFC QDDQSPICVV CREAQEHRMH RVLPLDEAAR EYKLKLEEDI KYLREEMMKT ETLQAKEEQT LTEWQERVKE RRERILEEFQ KVVLFLVEEE RRILQVLKKE EEDTLGKLQD SKASLDHQSR SLDLILLQLE ERSQQEPLQM LQDVKDTLNR KESFSVQYPE VVLPAAIKTL CRVPGQIEVL KSFQEDVMPD PSSAYPYLLL YESRQRRYLS PPPEGSAPYS KDRFVAYPCA VGQKSFSSGR HYWEVGMNLT GDALWALGVC RDNVSRKDRV LKSPENGFWV VQLSKGKKQL SLLPNSTLVT LTEPPSHMGI FLDFQAGEVS FYSVNDGSHL HSFSQAAFPG PLLPFFCLGA PKSGQMVIST VTMWVKG Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TRIM17 / RNF16 (TRIM17)

Alternative Name: Trim17 (TRIM17 Products)

Background: E3 ubiquitin-protein ligase TRIM17 (EC 2.3.2.27) (RING-type E3 ubiquitin transferase TRIM17) (Tripartite motif-containing protein 17),FUNCTION: E3 ubiquitin ligase that plays important roles in the regulation of neuronal apoptosis, selective autophagy or cell proliferation (PubMed:20559321, PubMed:22976837, PubMed:25215946). Stimulates the degradation of kinetochore ZW10 interacting protein ZWINT in a proteasome-dependent manner, leading to negative regulation of cell proliferation. Inhibits autophagic degradation of diverse known targets while contributing to autophagy of midbodies. Autophagy-inhibitory activity involves MCL1, which TRIM17 assembles into complexes with the key autophagy regulator BECN1 (By similarity). Controls neuronal apoptosis by mediating ubiquitination and degradation of MCL1 to initiate neuronal death (PubMed:22976837). In addition, regulates NFAT transcription factors NFATC3 and NFATC4 activities by preventing their nuclear localization, thus inhibiting their transcriptional activities (PubMed:25215946). Decreases TRIM41-mediated degradation of ZSCAN2 thereby stimulating alpha-synuclein/SNCA transcription in neuronal cells (PubMed:30485814). Prevents the E3 ubiquitin-ligase activity of TRIM28 and its interaction with anti-apoptotic BCL2A1, blocking TRIM28 from ubiquitinating BCL2A1 (By similarity). {ECO:0000250|UniProtKB:Q9Y577, ECO:0000269|PubMed:20559321, ECO:0000269|PubMed:22976837, ECO:0000269|PubMed:25215946, ECO:0000269|PubMed:30485814}.

Molecular Weight: 54.9 kDa

UniProt: Q7TPM3

AlphaFold: Q7TPM3