PELO Protein (AA 1-385) (His tag)

Catalog No. ABIN7558409

This Recombinant PELO protein is expressed in HEK-293 Cells.

Quick Overview for PELO Protein (AA 1-385) (His tag) (ABIN7558409)

Target: PELO (Pelota Homolog (PELO))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-385

Purification tag / Conjugate: This PELO protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Pelo Protein expressed in mammalian cells.

Sequence: MKLVRKDIEK DNAGQVTLVP EEPEDMWHTF NLVQVGDSLR ASTIRKVQTE SSTGSVGSNR VRTTLTLCVE AIDFDSQACQ LRVKGTNIQE NEYVKMGAYH TIELEPNRQF TLAKKQWDSV VLERIEQACD PAWSADVAAV VMQEGLAHVC LVTPSMTLTR AKVEVNIPRK RKGNCSQHDR ALERFYEQVV QAIQRHINFE VVKCVLVASP GFVREQFCDY MFQQAVKTDN KVLLENRSKF LQVHASSGHK YSLKEALCDP TVASRLSDTK AAGEVKALDD FYKMLQHEPD RAFYGLKQVE RANEALAIDT LLISDELFRH QDVATRSRYV RLVDSVKENA GTVRIFSSLH VSGEQLGQLT GVAAILRFPV PELSDQEDDS SSEED Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: PELO (Pelota Homolog (PELO))

Alternative Name: Pelo

Background: Protein pelota homolog,FUNCTION: Component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway. In the Pelota-HBS1L complex, PELO recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel. Following mRNA extraction from stalled ribosomes by the SKI complex, the Pelota-HBS1L complex promotes recruitment of ABCE1, which drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway. As part of the PINK1-regulated signaling, upon mitochondrial damage is recruited to the ribosome/mRNA-ribonucleoprotein complex associated to mitochondrial outer membrane thereby enabling the recruitment of autophagy receptors and induction of mitophagy. {ECO:0000250|UniProtKB:Q9BRX2}.

Molecular Weight: 43.3 kDa

UniProt: Q80X73

AlphaFold: Q80X73