Phospholipase D4 Protein (PLD4) (AA 1-503) (His tag)

Catalog No. ABIN7558514

This Recombinant Phospholipase D4 protein is expressed in HEK-293 Cells.

Quick Overview for Phospholipase D4 Protein (PLD4) (AA 1-503) (His tag) (ABIN7558514)

Target: Phospholipase D4 (PLD4)

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-503

Purification tag / Conjugate: This Phospholipase D4 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Pld4 Protein expressed in mammalian cells.

Sequence: MDKKKEHPEM RIPLQTAVEV SDWPCSTSHD PHSGLGMVLG MLAVLGLSSV TLILFLWQGA TSFTSHRMFP EEVPSWSWET LKGDAEQQNN SCQLILVESI PEDLPFAAGS PTAQPLAQAW LQLLDTARES VHIASYYWSL TGLDIGVNDS SSRQGEALLQ KFQQLLLRNI SVVVATHSPT LAKTSTDLQV LAAHGAQIRQ VPMKQLTGGV LHSKFWVVDG RHIYVGSANM DWRSLTQVKE LGAIIYNCSN LAQDLEKTFQ TYWVLGTPQA VLPKTWPRNF SSHINRFHPL RGPFDGVPTT AYFSASPPSL CPHGRTRDLD AVLGVMEGAR QFIYVSVMEY FPTTRFTHHA RYWPVLDNAL RAAALNKGVH VRLLVSCWFN TDPTMFAYLR SLQAFSNPSA GISVDVKVFI VPVGNHSNIP FSRVNHSKFM VTDKTAYVGT SNWSEDYFSH TAGVGLIVSQ KTPRAQPGAT TVQEQLRQLF ERDWSSHYAM DLDRQVPSQD CVW Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Phospholipase D4 (PLD4)

Alternative Name: Pld4

Background: 5'-3' exonuclease PLD4 (EC 3.1.16.1) (Choline phosphatase 4) (Phosphatidylcholine-hydrolyzing phospholipase D4) (Phospholipase D4) (PLD 4),FUNCTION: 5'->3' DNA exonuclease which digests single-stranded DNA (ssDNA) (PubMed:30111894). Regulates inflammatory cytokine responses via the degradation of nucleic acids, by reducing the concentration of ssDNA able to stimulate TLR9, a nucleotide-sensing receptor (PubMed:30111894). Involved in phagocytosis of activated microglia (PubMed:22102906). {ECO:0000269|PubMed:22102906, ECO:0000269|PubMed:30111894}.

Molecular Weight: 56.2 kDa

UniProt: Q8BG07

AlphaFold: Q8BG07