POMT2 Protein (AA 1-820) (His tag)

Catalog No. ABIN7558573

Product Details

Target: POMT2 (Protein-O-Mannosyltransferase 2 (POMT2))

Protein Type: Recombinant

Protein Characteristics: AA 1-820

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This POMT2 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Pomt2 Protein expressed in mammalian cells.

Sequence: MLYASGRLLA ARAATTLSAP PRARGPALRG KRRELQIPWH LETPSYDPLT GQRTRPGVPP ARRVILRKGR MPPAIGGGLA GSELRPRRGR CVPQAARAVS RDVVPQAAAR KLKRPAWSSR RFQAAGWWAT LAVVTLLSFA TRFHRLDQPA HICWDETHFG KMGSYYINRT FFFDVHPPLG KMLIGLAGYL SGYDGTFLFQ KPGDRYEHHS YMGMRGFCAF LGSWLIPFAY LTVLDLSKSF PAALLTAALL TFDTGCLTLS QYILLDPILM FFIMAAMLSM VKYNSCANRP FSAPWWFWLS LTGISLAGAL GVKFVGLFII VQVGLNTISD LWHLFGDLSL SLVTVGKHLT ARILCLIVLP LVLYVTIFAV HVMVLNKSGP GDGFFSSAFQ ARLSGNSLHN ASIPEHLAYG SVITVKNLRM AIGYLHSHRH LYPEGIGARQ QQVTTYLHKD YNNLWIIKKY NANTDPLDPS FPVEFVRHGD IIRLEHKETT RNLHSHYHEA PLTRKHYQVT GYGINGTGDS NDFWRIEVVN RKFGNRIKVL RSRIRLIHLV TGCVLGSSGK ILPKWGWEQL EVTCTPYLKE TTNSIWNIEE HINPKLPNIS LDVLQPSFPE ILLESHMVMI RGNNGLKPKD NEFTSKPWHW PINYQGLRFS GANDTDFRVY LLGNPVVWWL NLVSIVLYLL SGSTIAVAMQ RGIQLPAELQ GLTKVLLRGG GQLLLGWMLH YFPFFLMGRI LYFHHYFPAM LFSSMLTGIL WDTLLRLCAW GLAPSPLGRR IHAVGILSLL LTTAYSFYLF HPLAYGMVGP LAQEPESPMA GLRWLESWDF Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: POMT2 (Protein-O-Mannosyltransferase 2 (POMT2))

Alternative Name: Pomt2 (POMT2 Products)

Background: Protein O-mannosyl-transferase 2 (EC 2.4.1.109) (Dolichyl-phosphate-mannose--protein mannosyltransferase 2),FUNCTION: Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. Coexpression of both POMT1 and POMT2 is necessary for enzyme activity, expression of either POMT1 or POMT2 alone is insufficient. Essentially dedicated to O-mannosylation of alpha-DAG1 and few other proteins but not of cadherins and protocaherins. {ECO:0000250|UniProtKB:Q9UKY4}.

Molecular Weight: 92.4 kDa

UniProt: Q8BGQ4

AlphaFold: Q8BGQ4