GEMIN8 Protein (AA 1-238) (His tag)

Catalog No. ABIN7558633

Product Details

Target: GEMIN8 (Gem (Nuclear Organelle) Associated Protein 8 (GEMIN8))

Protein Type: Recombinant

Protein Characteristics: AA 1-238

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This GEMIN8 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Gemin8 Protein expressed in mammalian cells.

Sequence: MASNWRASAS WYSHPVYARY WQHYHHAMLW MQGHQNAYRK FRDSYFTSPW LFPHGALPWN SPAYEAGHPW DSQGQHMAQQ ESPYRVSHPK SPGQPLRNSS RTQASTRGNE ARCEEEELES DSDDEVECDL SNMEITEELR QYFAQTERHR EERRRQQQLD AERLNDYVNA DHGLYFNHRR SLEPPSEKPW ERRQAEMKRL YGNSAPKILA METAVQLSFD KHCDRKQPKY WPVIPLKF Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: GEMIN8 (Gem (Nuclear Organelle) Associated Protein 8 (GEMIN8))

Alternative Name: Gemin8 (GEMIN8 Products)

Background: Gem-associated protein 8 (Gemin-8) (Protein FAM51A1),FUNCTION: The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Binding of snRNA inside 5Sm triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP (By similarity). {ECO:0000250|UniProtKB:Q9NWZ8}.

Molecular Weight: 28.4 kDa

UniProt: Q8BHE1

AlphaFold: Q8BHE1

Pathways: Ribonucleoprotein Complex Subunit Organization