NIM1 Protein (AA 1-436) (His tag)
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- Target See all NIM1 Proteins
- NIM1 (Serine/threonine-Protein Kinase NIM1 (NIM1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-436
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This NIM1 protein is labelled with His tag.
- Purpose
- Predefined custom protein recombinant Nim1k Protein expressed in mammalian cells.
- Sequence
- MTAVFVNGGG LVNTHCDRWE RRDSVESSCQ TEGGKDSEED QQRQLTPFEK LTQDMCQDEK VVREITLGKR IGFYRIRGEI GSGNFSQVKL GIHSLTKEKV AIKILDKTKL DQKTQRLLSR EISSMEKLHH PNIVRLYEVV ETLSKLHLVM EYAGGGELFG KISTEGKLSE PESKLIFSQI LSAVKHMHEN QIIHRDLKAE NVFYTSRTCV KVGDFGFSTV SKKGEMLNTF CGSPPYAAPE LFRDQHYVGV YVDIWALGVL LYFMVTGTMP FRAETVAKLK KSILEGTYTI PQHVSEPCHR LIRGVLQPTP TERYGINYIM SNEWMRGVPY PTPLEPFQLD PKHLSETSTL KEEENEVKST LEHLGITDEH IRNNQGRDAR SSITGVYRII LHRVQRRKAL ESVPIMILPE PKERDLKKGS RIYRGIRHTS KFCSIL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Serine/threonine-Protein Kinase NIM1 (NIM1) (AA 1-436) protein (His tag)
custom-made NIM1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Serine/threonine-Protein Kinase NIM1 (NIM1) (AA 1-436) protein (GST tag)NIM1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Serine/threonine-Protein Kinase NIM1 (NIM1) (AA 1-436), (full length) protein (His-SUMO Tag)NIM1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Serine/threonine-Protein Kinase NIM1 (NIM1) protein (Myc-DYKDDDDK Tag)NIM1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- NIM1 (Serine/threonine-Protein Kinase NIM1 (NIM1))
- Alternative Name
- Nim1k (NIM1 Products)
- Background
- Serine/threonine-protein kinase NIM1 (EC 2.7.11.1) (NIM1 serine/threonine-protein kinase)
- Molecular Weight
- 49.8 kDa
- UniProt
- Q8BHI9
- AlphaFold
- Q8BHI9
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