ENGASE Protein (AA 1-734) (His tag)

Catalog No. ABIN7558991

Product Details

Target: ENGASE (Endo-beta-N-Acetylglucosaminidase (ENGASE))

Protein Type: Recombinant

Protein Characteristics: AA 1-734

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This ENGASE protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Engase Protein expressed in mammalian cells.

Sequence: METSSVLTRG AARQRSPAAP EKQARDQTER RPGRRRQGRR INEDQEEEAV FREVVSFTPD PLPARYYDKD TTRPISFYLS TLEELLAWTP LMEDGFNVAL EPLVCRRPPL SSPRPRTLLC HDMMGGYLED RFIQGSEVQN PYSFYHWQYI DIFVYFSHHT VTIPPVCWTN AAHRHGVCVL GTFITEWQEG GRLCEAFLAG DEPSFQAVAD RLVQIAQFFR FDGWLINIEN SLTPAAVRNT PLFLQYLTAQ LHQQVPGGLV LWYDSVVQSG QLKWQDELND QNRVFFDSCD GFFTNYNWRE DHLQRMVAQA GERLADVYVG VDVFARSNVV GGRFDTDKSL ELIRKHGFSA ALFAPGWVYE CLEKSDFFQN QDKFWSLLER FLPTHSICSL PFVTSFCLGL GTRRVCYGKE QAVGPWYHPS AQETQPLFGE HKLAGDSRGW VKTHCCLTDA WHGGSSLLLR GLIPPEVDSV AVRLFSLHIP VPPKVFLSMV YKFEGSTDVQ VALELTTGDA SSCHVGGMLV LNETGSRHSP RPLRVPPTRL ARWASSCGQQ LSGGWIQRCY EVNLRGCLLQ DLLVSFSRPP GSREEESFIC RLGEIQVVDA SSLLAPLPRV QNVTISQIRW LPLITGSEGL PTRLLLSCTL HWSYLLLRAR CFRIHCWKRT GSSSSVAESP ETEKPTFLGL AFANQYRVVD LAVEAAGFGQ DGRVEFLVEP VPREGFLVPQ AEWGKAVLLF SVPQ Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ENGASE (Endo-beta-N-Acetylglucosaminidase (ENGASE))

Alternative Name: Engase (ENGASE Products)

Background: Cytosolic endo-beta-N-acetylglucosaminidase (ENGase) (EC 3.2.1.96),FUNCTION: Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol (By similarity). {ECO:0000250}.

Molecular Weight: 82.9 kDa

UniProt: Q8BX80

AlphaFold: Q8BX80