TRAF3IP3 Protein (AA 1-513) (His tag)

Catalog No. ABIN7559071

Product Details

Target: TRAF3IP3 (TRAF3 Interacting Protein 3 (TRAF3IP3))

Protein Type: Recombinant

Protein Characteristics: AA 1-513

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This TRAF3IP3 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Traf3ip3 Protein expressed in mammalian cells.

Sequence: MISSDSRSSP GLARWAESYE AKCERRQETR ENRRRRRNET TCRQPGKVLR TQHKERLQGA RQLQFLKRRN LEEEKKGQAR EQGPSSKTDG GTGQVSILKE SLPGANKASF PGQQETGISS EVFPALHHSS SGIQRDLGGH HASHGRAFPP QDSDIKKPHR QHRGTQTKAE EALPTIKNDA SQQTNCGVAV LDKDIIQLSE YLKEALHREL ILKKKMVILQ DLLPALIRAS DSSWKGQLNE DKLKGKLRSL ENQLYTCLQK HSPWGMKKVL LEMEDQRSSY EQKAKASLQK VLEEKMCAEQ QLQRAQLSLA LAEQKCQEWK SQYEALKEDW RTLGDQHREL ESQLHVLQSK LQGADSRDSQ MSQALQLLEN EHQELQTKLE SLQGDGEQQS SETQDLQDQL KKSEEEKQAL VSKVQQLQSL LQNQSLQLQE QEKLLKKDQG LPVWNPKLSL DEVKPEGTRK EKEEELRDQL QKETFQLQVK ENELQCGQWL PVLMVVIATA LAVFLANKGN LVI Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TRAF3IP3 (TRAF3 Interacting Protein 3 (TRAF3IP3))

Alternative Name: Traf3ip3 (TRAF3IP3 Products)

Background: TRAF3-interacting JNK-activating modulator (TRAF3-interacting protein 3),FUNCTION: Adapter protein that plays essential roles in both innate and adaptive immunity. Plays a crucial role in the regulation of thymocyte development (PubMed:26195727). Mechanistically, mediates TCR-stimulated activation through recruiting MAP2K1/MEK1 to the Golgi and, thereby, facilitating the interaction of MAP2K1/MEK1 with its activator BRAF (PubMed:26195727). Also plays an essential role in regulatory T-cell stability and function by recruiting the serine-threonine phosphatase catalytic subunit (PPP2CA) to the lysosome, thereby facilitating the interaction of PP2Ac with the mTORC1 component RPTOR and restricting glycolytic metabolism (PubMed:30115741). Positively regulates TLR4 signaling activity in macrophage-mediated inflammation by acting as a molecular clamp to facilitate LPS-induced translocation of TLR4 to lipid rafts (PubMed:30115741). In response to viral infection, facilitates the recruitment of TRAF3 to MAVS within mitochondria leading to IRF3 activation and interferon production (PubMed:30115741). However, participates in the maintenance of immune homeostasis and the prevention of overzealous innate immunity by promoting 'Lys-48'-dependent ubiquitination of TBK1 (By similarity). {ECO:0000250|UniProtKB:Q9Y228, ECO:0000269|PubMed:26195727, ECO:0000269|PubMed:30115741}.

Molecular Weight: 58.6 kDa

UniProt: Q8C0G2

AlphaFold: Q8C0G2