TRPT1 Protein (AA 1-249) (His tag)
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- Target See all TRPT1 Proteins
- TRPT1 (tRNA Phosphotransferase 1 (TRPT1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-249
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TRPT1 protein is labelled with His tag.
- Purpose
- Made-to-order recombinant Trpt1 Protein expressed in mammalian cells.
- Sequence
- MNAPGGRRKE GRRTHRPREQ DRNVQLSKAL SYALRHGALK LGLPMRADGF VPLQALLQLP QFHSFSIEDV QLVVNTNEKQ RFTLQPGEPS TGLLIRANQG HSLQVPELEL TPLETPQALP LTLVHGTFWK HWPSILLKGL SRQGRTHIHL ASGLPGDPGV ISGIRPNCEV AVFIDGPLAL TDGIPFFCSA NGVILTPGNA EGFLLPKYFK EALQLRPTRK PLSLAGDKET ETQSGPKLSS RGGRRKIQQ Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
- Top Product
- Discover our top product TRPT1 Protein
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tRNA Phosphotransferase 1 (TRPT1) (AA 1-253) protein (His tag)
custom-made TRPT1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
tRNA Phosphotransferase 1 (TRPT1) (AA 1-253) protein (His tag)TRPT1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
tRNA Phosphotransferase 1 (TRPT1) (AA 1-204) protein (GST tag)TRPT1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
tRNA Phosphotransferase 1 (TRPT1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)TRPT1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- TRPT1 (tRNA Phosphotransferase 1 (TRPT1))
- Alternative Name
- Trpt1 (TRPT1 Products)
- Background
- TRNA 2'-phosphotransferase 1 (mTPT1) (EC 2.7.1.160),FUNCTION: Catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate. {ECO:0000269|PubMed:9826666}.
- Molecular Weight
- 27.4 kDa
- UniProt
- Q8K3A2
- AlphaFold
- Q8K3A2
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