ABHD11 Protein (AA 1-307) (His tag)

Catalog No. ABIN7559569

This Recombinant ABHD11 protein is produced in HEK-293 Cells.

Quick Overview for ABHD11 Protein (AA 1-307) (His tag) (ABIN7559569)

Target: ABHD11 (Abhydrolase Domain Containing 11 (ABHD11))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-307

Purification tag / Conjugate: This ABHD11 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Abhd11 Protein expressed in mammalian cells.

Sequence: MLRWARAWRV PRGVLGASSP RRLAVPVTFC SSRSSGQENA DLRPLPLSYN LLDGDATLPA IVFLHGLFGS KTNFNSLAKA MVQRTGRRVL TVDARNHGDS PHSPDASYEA MSQDLQGLLP QLGLVPCVLV GHSMGGKTAM LLALQRPDVV ERLVVVDISP VGTTPGSHIG AFIAAMKAVE IPEKVPHSQA RKLADKQLSS VVKEAGIRQF LLTNLVEVGG RFSWRLNLDT LAQHLDKIMT FPQQREPYSG PTLFLLGGNS TYVQPSHHSE IRRLFPQAQI QTVPNAGHWV HSDKPQDFMD AVTSFLA Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ABHD11 (Abhydrolase Domain Containing 11 (ABHD11))

Alternative Name: Abhd11

Background: Sn-1-specific diacylglycerol lipase ABHD11 (EC 3.1.1.116) (Alpha/beta hydrolase domain-containing protein 11) (Abhydrolase domain-containing protein 11) (Williams-Beuren syndrome chromosomal region 21 protein homolog),FUNCTION: Catalyzes the hydrolysis of diacylglycerol in vitro and may function as a key regulator in lipid metabolism, namely by regulating the intracellular levels of diacylglycerol (By similarity). 1,2-diacyl-sn-glycerols are the preferred substrate over 1,3-diacyl-sn-glycerols. The enzyme hydrolyzes stearate in preference to palmitate from the sn-1 position of 1,2-diacyl-sn-glycerols (By similarity). Maintains the functional lipoylation of the 2-oxoglutarate dehydrogenase complex (OGDHc) through its interaction with the OGDHc by preventing the formation of lipoyl adducts (By similarity). In addition, is also required for the expansion and differentiation of embryonic stem cells (ESCs) (PubMed:32733886). {ECO:0000250|UniProtKB:Q3SZ73, ECO:0000250|UniProtKB:Q8NFV4, ECO:0000269|PubMed:32733886}.

Molecular Weight: 33.6 kDa

UniProt: Q8K4F5

AlphaFold: Q8K4F5