DDX54 Protein (AA 1-874) (His tag)

Catalog No. ABIN7559578

Product Details

Target: DDX54 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 54 (DDX54))

Protein Type: Recombinant

Protein Characteristics: AA 1-874

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This DDX54 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Ddx54 Protein expressed in mammalian cells.

Sequence: MAAGRRVGPG PPSRPTMAPW KKKRLRKRRT GASQGRDSDS DDGEFEIQAE DDARARKLGP GRALPSFPTS ECVSDVEPDT REMVRAQNKK KKKSGGFQSM GLSYPVFKGI MKKGYKVPTP IQRKTIPVIL DGKDVVAMAR TGSGKTACFL LPMFERLKAR SAQTGARALI LSPTRELALQ TMKFTKELGK FTGLKTALIL GGDKMEDQFA ALHENPDIII ATPGRLVHVA VEMNLKLQSV EYVVFDEADR LFEMGFAEQL QEIIGRLPGG HQTVLFSATL PKLLVEFARA GLTEPVLIRL DVDSKLNEQL KTSFLLVRED TKAAVLLYLL QNVVRPQDQT VVFVATKHHA EYLTELLMGQ GVSCAHIYSA LDQTARKINL AKFTHNKCST LIVTDLAARG LDIPLLDNVI NYSFPAKGKL FLHRVGRVAR AGRSGTAYSL VAPDEVPYLL DLHLFLGRSV TLARPCEEPS VADAVGRDGV LGRVPQSVVD DEDSSLQTAM GASLDLQGLH RVANNAQQQY VRSRPAPSPE SIKRAKELDL AELGLHPLFS SCFEEGELQR LRLVDSIKNY RTRTTIFEIN ASSKDPSSQM MRAKRQRDRK AVASFQQRRQ ERQEGPADPA PQRELPQEEE EEMVETVEGV FTEVVGQKRP RPGPSQGAKR RRMETRQRDQ EFYVPYRPKD FDSERGLSVS GAGGAFEQQV AGAVLDLMGD EAQNMSRGQQ QLKWDRKKKR FVGQSGQEDK KKIKTESGRF ISSSYKRDLY QKWKQKQKID DRDSEEEGPS NQRGPGPRRG GKRGRSQGTS QPRASSVPAG RMRSELKTKE QILKQRRQAQ KQRFLQRGGL KQLSARNRRR AQELRQGAFG RGAPSRKGKM RKRM Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: DDX54 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 54 (DDX54))

Alternative Name: Ddx54 (DDX54 Products)

Background: ATP-dependent RNA helicase DDX54 (EC 3.6.4.13) (DEAD box protein 54),FUNCTION: Has RNA-dependent ATPase activity. Represses the transcriptional activity of nuclear receptors (By similarity). {ECO:0000250}.

Molecular Weight: 97.7 kDa

UniProt: Q8K4L0

AlphaFold: Q8K4L0

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway