GGA1 Protein (AA 1-635) (His tag)

Catalog No. ABIN7559631

Recombinant GGA1 protein expressed in HEK-293 Cells.

Quick Overview for GGA1 Protein (AA 1-635) (His tag) (ABIN7559631)

Target: GGA1 (Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 1 (GGA1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-635

Purification tag / Conjugate: This GGA1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Gga1 Protein expressed in mammalian cells.

Sequence: MEPAMEPETL EARINRATNP LNKELNWASI NSFCEQLNED FEGPPLATRL LAHKIQSPQE WEAIQALTVL ETCMKSCGKR FHDEVGKFRF LNELIKVVSP KYLGSRTSEK VKSKILELLY SWTVCLPEEV KIAEAYQMLK KQGIVKSDPK LPEDAIFPLP PPRPKNVIFE DEEKSKMLAR LLKSSHPEDL RAANKLIKEM VQEDQKRMEK ISKRVNAIEE VNNNVKLLTE MVMSHSQGAA SSSSEDLMKE LYQRCERMRP TLFRLASDTE DNDEALAEIL QANDNLTQVI NLYKQLVRGE EVNGDATASS IPGSTSALLD LSGLDLPPPG TTQPATPTRP GNQSSPEQLS ASVSLLDDEL MSLGLSDPTP PSGTSSDSVG WDNFQSSDGT ESSVPPPAQA PSMDCRPPAQ APPPTSSGLD DLDLLGKTLM QQALPPEAQQ VRWEKQQPAP RLTLRDLQSK SSSPSPGAAS LLHTTSPEPP GPPPQATPTE FSLTSITVPL ESIKPSSILP VTVYDQHGFR VLFHFARDPL PGRSDVLVVV VSMLSTAPQP IRNIVFQSAV PKVMKVRLQP PSGTELPAFN PIVHPSAITQ VLLLANPQKE KVRLRYKLIF TMGDQTYNEM GDVDQFPPPE TWGSL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: GGA1 (Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 1 (GGA1))

Alternative Name: Gga1

Background: ADP-ribosylation factor-binding protein GGA1 (Gamma-adaptin-related protein 1) (Golgi-localized, gamma ear-containing, ARF-binding protein 1),FUNCTION: Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes (PubMed:25405894). Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Mediates export of the GPCR receptor ADRA2B to the cell surface (By similarity). Required for targeting PKD1:PKD2 complex from the trans-Golgi network to the cilium membrane (PubMed:25405894). Regulates retrograde transport of proteins such as phosphorylated form of BACE1 from endosomes to the trans-Golgi network (By similarity). {ECO:0000250|UniProtKB:Q9UJY5, ECO:0000269|PubMed:25405894}.

Molecular Weight: 70.0 kDa

UniProt: Q8R0H9

AlphaFold: Q8R0H9