Mucolipin 3 Protein (Mcoln3) (AA 1-553) (His tag)
custom-made
Mcoln3
Origin: Mouse
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all Mucolipin 3 (Mcoln3) products
- Mucolipin 3 (Mcoln3)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-553
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Mucolipin 3 protein is labelled with His tag.
- Purpose
- Custom-made recombinant Mcoln3 Protein expressed in mammalian cells.
- Sequence
- MANPEVLVSS CRARQDESPC TFHPSSSPSE QLLLEDQMRR KLKFFFMNPC EKFWARGRKP WKLAIQILKI AMVTIQLVLF GLSNQMVVAF KEENTIAFKH LFLKGYMDRM DDTYAVYTQS EVYDQIIFAV TQYLQLQNIS VGNHAYENKG TKQSAMAICQ HFYRQGTICP GNDTFDIDPE VETECFLVEP DEASHLGTPG ENKLNLSLDF HRLLTVELQF KLKAINLQTV RHQELPDCYD FTLTITFDNK AHSGRIKISL DNDISIKECK DWHVSGSIQK NTHYMMIFDA FVILTCLASL VLCARSVIRG LQLQQEFVNF FLLHYKKEVS ASDQMEFING WYIMIIISDI LTIVGSVLKM EIQAKSLTSY DVCSILLGTS TMLVWLGVIR YLGFFAKYNL LILTLQAALP NVMRFCCCAA MIYLGYCFCG WIVLGPYHEK FRSLNRVSEC LFSLINGDDM FSTFAKMQQK SYLVWLFSRV YLYSFISLFI YMILSLFIAL ITDTYETIKH YQQDGFPETE LRKFIAECKD LPNSGKYRLE DDPPGSLLCC CKK Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Mucolipin 3 (Mcoln3) (AA 1-553) protein (His tag)
custom-made Mcoln3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- Mucolipin 3 (Mcoln3)
- Alternative Name
- Mcoln3 (Mcoln3 Products)
- Background
- Mucolipin-3 (Transient receptor potential channel mucolipin 3) (TRPML3),FUNCTION: Nonselective ligand-gated cation channel probably playing a role in the regulation of membrane trafficking events. Acts as a Ca(2+)-permeable cation channel with inwardly rectifying activity (PubMed:17989217). Mediates release of Ca(2+) from endosomes to the cytoplasm, contributes to endosomal acidification and is involved in the regulation of membrane trafficking and fusion in the endosomal pathway (By similarity). Does not seem to act as mechanosensory transduction channel in inner ear sensory hair cells. Proposed to play a critical role at the cochlear stereocilia ankle-link region during hair-bundle growth (PubMed:18801844). Involved in the regulation of autophagy. Through association with GABARAPL2 may be involved in autophagosome formation possibly providing Ca(2+) for the fusion process (PubMed:24269818). Through a possible and probably tissue-specific heteromerization with MCOLN1 may be at least in part involved in many lysosome-dependent cellular events. Possible heteromeric ion channel assemblies with TRPV5 show pharmacological similarity with TRPML3 (By similarity). {ECO:0000250|UniProtKB:Q8TDD5, ECO:0000269|PubMed:17989217, ECO:0000269|PubMed:18801844, ECO:0000269|PubMed:24269818}.
- Molecular Weight
- 63.7 kDa
- UniProt
- Q8R4F0
- Pathways
- Sensory Perception of Sound
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