Mucolipin 3 Protein (Mcoln3) (AA 1-553) (Strep Tag)

Catalog No. ABIN3126789

This Recombinant Mucolipin 3 protein is produced in Cell-free protein synthesis (CFPS).

Quick Overview for Mucolipin 3 Protein (Mcoln3) (AA 1-553) (Strep Tag) (ABIN3126789)

Target: Mucolipin 3 (Mcoln3)

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-553

Purification tag / Conjugate: This Mucolipin 3 protein is labelled with Strep Tag.

Sequence: MANPEVLVSS CRARQDESPC TFHPSSSPSE QLLLEDQMRR KLKFFFMNPC EKFWARGRKP WKLAIQILKI AMVTIQLVLF GLSNQMVVAF KEENTIAFKH LFLKGYMDRM DDTYAVYTQS EVYDQIIFAV TQYLQLQNIS VGNHAYENKG TKQSAMAICQ HFYRQGTICP GNDTFDIDPE VETECFLVEP DEASHLGTPG ENKLNLSLDF HRLLTVELQF KLKAINLQTV RHQELPDCYD FTLTITFDNK AHSGRIKISL DNDISIKECK DWHVSGSIQK NTHYMMIFDA FVILTCLASL VLCARSVIRG LQLQQEFVNF FLLHYKKEVS ASDQMEFING WYIMIIISDI LTIVGSVLKM EIQAKSLTSY DVCSILLGTS TMLVWLGVIR YLGFFAKYNL LILTLQAALP NVMRFCCCAA MIYLGYCFCG WIVLGPYHEK FRSLNRVSEC LFSLINGDDM FSTFAKMQQK SYLVWLFSRV YLYSFISLFI YMILSLFIAL ITDTYETIKH YQQDGFPETE LRKFIAECKD LPNSGKYRLE DDPPGSLLCC CKK
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Mucolipin 3 (Mcoln3)

Alternative Name: Mcoln3

Background: Mucolipin-3 (Transient receptor potential channel mucolipin 3) (TRPML3),FUNCTION: Nonselective ligand-gated cation channel probably playing a role in the regulation of membrane trafficking events. Acts as a Ca(2+)-permeable cation channel with inwardly rectifying activity (PubMed:17989217). Mediates release of Ca(2+) from endosomes to the cytoplasm, contributes to endosomal acidification and is involved in the regulation of membrane trafficking and fusion in the endosomal pathway (By similarity). Does not seem to act as mechanosensory transduction channel in inner ear sensory hair cells. Proposed to play a critical role at the cochlear stereocilia ankle-link region during hair-bundle growth (PubMed:18801844). Involved in the regulation of autophagy. Through association with GABARAPL2 may be involved in autophagosome formation possibly providing Ca(2+) for the fusion process (PubMed:24269818). Through a possible and probably tissue-specific heteromerization with MCOLN1 may be at least in part involved in many lysosome-dependent cellular events. Possible heteromeric ion channel assemblies with TRPV5 show pharmacological similarity with TRPML3 (By similarity). {ECO:0000250|UniProtKB:Q8TDD5, ECO:0000269|PubMed:17989217, ECO:0000269|PubMed:18801844, ECO:0000269|PubMed:24269818}.

Molecular Weight: 63.7 kDa

UniProt: Q8R4F0

Pathways: Sensory Perception of Sound