SLC39A8 Protein (AA 1-462) (His tag)

Catalog No. ABIN7560007

This Recombinant SLC39A8 protein is produced in HEK-293 Cells.

Quick Overview for SLC39A8 Protein (AA 1-462) (His tag) (ABIN7560007)

Target: SLC39A8 (Solute Carrier Family 39 (Zinc Transporter), Member 8 (SLC39A8))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-462

Purification tag / Conjugate: This SLC39A8 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Slc39a8 Protein expressed in mammalian cells.

Sequence: MAPGRAVAGL LLLAATSLGH PSEGPELAFS EDVLSVFGAN RSLSAAQLGR LLERLGAASQ QGALDLGQLH FNQCLSAEDI FSLHGFSNVT QITSSNFSAI CPAILQQLNF HPCEDLRKHN AKPSLSEVWG YGFLSVTIIN LASLLGLILT PLIKKSYFPK ILTYFVGLAI GTLFSNAIFQ LIPEAFGFNP KIDNYVEKAV AVFGGFYMLF FVERTLKMLL KTYGQNDHTH FRNDDFGSKE KTHQPKTLPL PAVNGVTCYA NPAVTEPNGH IHFDTVSVVS LQDGKTEPSS CTCLKGPKLS EIGTIAWMIT LCDALHNFID GLAIGASCTL SLLQGLSTSI AILCEEFPHE LGDFVILLNA GMSTRQALLF NFLSACSCYV GLAFGILVGN NFAPNIIFAL AGGMFLYISL ADMFPEMNDM LREKVTGRQT DFTFFMIQNA GMLTGFTAIL LITLYAGDIE LQ Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SLC39A8 (Solute Carrier Family 39 (Zinc Transporter), Member 8 (SLC39A8))

Alternative Name: Slc39a8

Background: Metal cation symporter ZIP8 (Solute carrier family 39 member 8) (Zrt- and Irt-like protein 8) (ZIP-8),FUNCTION: Electroneutral divalent metal cation:bicarbonate symporter of the plasma membrane mediating the cellular uptake of zinc and manganese, two divalent metal cations important for development, tissue homeostasis and immunity (PubMed:16638970, PubMed:18037372, PubMed:22563477, PubMed:29337306). Transports an electroneutral complex composed of a divalent metal cation and two bicarbonate anions or alternatively a bicarbonate and a selenite anion (PubMed:16638970, PubMed:18037372, PubMed:22563477, PubMed:27166256). Thereby, it also contributes to the cellular uptake of selenium, an essential trace metal and micronutrient (PubMed:27166256). Also imports cadmium a non-essential metal which is cytotoxic and carcinogenic (PubMed:15722412, PubMed:16638970, PubMed:17108009, PubMed:18037372, PubMed:24529376). May also transport iron and cobalt through membranes (PubMed:16638970, PubMed:22898811, PubMed:24529376). Through zinc import, indirectly regulates the metal-dependent transcription factor MTF1 and the expression of some metalloproteases involved in cartilage catabolism and also probably heart development (PubMed:24529376, PubMed:29337306). Also indirectly regulates the expression of proteins involved in cell morphology and cytoskeleton organization (PubMed:29927450). Indirectly controls innate immune function and inflammatory response by regulating zinc cellular uptake which in turn modulates the expression of genes specific of these processes (PubMed:23403290). Protects, for instance, cells from injury and death at the onset of inflammation (By similarity). By regulating zinc influx into monocytes also directly modulates their adhesion to endothelial cells and arteries (PubMed:30015240). Reclaims manganese from the bile at the apical membrane of hepatocytes, thereby regulating the activity of the manganese-dependent enzymes through the systemic levels of the nutrient (PubMed:28481222). Also participates in manganese reabsorption in the proximal tubule of the kidney (By similarity). By mediating the extracellular uptake of manganese by cells of the blood-brain barrier, may also play a role in the transport of the micronutrient to the brain. With manganese cellular uptake also participates in mitochondrial proper function (By similarity). Finally, also probably functions intracellularly, translocating zinc from lysosome to cytosol to indirectly enhance the expression of specific genes during TCR-mediated T cell activation (By similarity). {ECO:0000250|UniProtKB:Q9C0K1, ECO:0000269|PubMed:15722412, ECO:0000269|PubMed:16638970, ECO:0000269|PubMed:17108009, ECO:0000269|PubMed:18037372, ECO:0000269|PubMed:22563477, ECO:0000269|PubMed:22898811, ECO:0000269|PubMed:23403290, ECO:0000269|PubMed:24529376, ECO:0000269|PubMed:27166256, ECO:0000269|PubMed:28481222, ECO:0000269|PubMed:29337306, ECO:0000269|PubMed:29927450, ECO:0000269|PubMed:30015240}.

Molecular Weight: 50.1 kDa

UniProt: Q91W10

AlphaFold: Q91W10