SLC15A4 Protein (AA 1-574) (His tag)

Catalog No. ABIN7560021

Recombinant SLC15A4 protein expressed in HEK-293 Cells.

Quick Overview for SLC15A4 Protein (AA 1-574) (His tag) (ABIN7560021)

Target: SLC15A4 (Solute Carrier Family 15 (H+/Peptide Transporter), Member 4 (SLC15A4))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-574

Purification tag / Conjugate: This SLC15A4 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Slc15a4 Protein expressed in mammalian cells.

Sequence: MEGERAPLLG SRRPAVSAAS AVFAGRRAAC GAVLLAELLE RAAFYGVTAN LVLFLNGAPF DWEGAQASQA LLLFMGLTYL GSPFGGWLAD ARLGRARAIL LSLALYLLGL LAFPLLAAPR SRSFLCGDPR PELVRNCSAP FPNGSASCPE NAARRCAPAT FAGLVLVGLG VATVKANITP FGADQVKDRG PEATRRFFNW FYWSINLGAI LSLGGIAYIQ QNVSFFTGYL IPTVCVAIAF LVFLCGQSVF ITKPPDGSAF TDMFRILTYS CCSQRGGQRR SGEGLGVFQQ SSKHSLFDSC KMSRGGPFTE DKVEDVKALV KIVPVFLALI PYWTVYFQMQ TTYALQSLHL KIPEISSITT THHTLPAAWL TMFDAVLILL LIPLKDKLVD PVLRRHGLLP SSLKRIAVGM FFVMCSAFAA GILESKRLDL VKEKTINQTI GGVVYHAADL PIWWQIPQYV LIGISEIFAS IAGLEFAYSA APKSMQSAIM GLFFFFSGIG SFVGSGLLAL VSLKAIGWMS SHTDFGNINS CHLHYYFFLL AAIQGATLLL FLIVSVKYDR QRARTDGGPA STRT Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SLC15A4 (Solute Carrier Family 15 (H+/Peptide Transporter), Member 4 (SLC15A4))

Alternative Name: Slc15a4

Background: Solute carrier family 15 member 4 (Peptide/histidine transporter 1),FUNCTION: Proton-coupled amino-acid transporter that mediates the transmembrane transport of L-histidine and some di- and tripeptides from inside the lysosome to the cytosol, and plays a key role in innate immune response (PubMed:19570976, PubMed:21277849, PubMed:25238095, PubMed:27845049, PubMed:29305823). Able to transport a variety of di- and tripeptides, including carnosine and some peptidoglycans (PubMed:29784761). Transporter activity is  pH -dependent and maximized in the acidic lysosomal environment (By similarity). Involved in the detection of microbial pathogens by toll-like receptors (TLRs) and NOD-like receptors (NLRs), probably by mediating transport of bacterial peptidoglycans across the endolysosomal membrane: catalyzes the transport of certain bacterial peptidoglycans, such as muramyl dipeptide (MDP), the NOD2 ligand, and L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate (tri-DAP), the NOD1 ligand (PubMed:19570976, PubMed:23028315, PubMed:25238095, PubMed:24695226, PubMed:29784761). Required for TLR7, TLR8 and TLR9-mediated type I interferon (IFN-I) productions in plasmacytoid dendritic cells (pDCs) (PubMed:21045126, PubMed:23382217, PubMed:25238095, PubMed:30262916). Independently of its transporter activity, also promotes the recruitment of innate immune adapter TASL to endolysosome downstream of TLR7, TLR8 and TLR9: TASL recruitment leads to the specific recruitment and activation of IRF5 (By similarity). Required for isotype class switch recombination to IgG2c isotype in response to TLR9 stimulation (PubMed:25310967). Required for mast cell secretory-granule homeostasis by limiting mast cell functions and inflammatory responses (PubMed:29155995). {ECO:0000250|UniProtKB:O09014, ECO:0000250|UniProtKB:Q8N697, ECO:0000269|PubMed:19570976, ECO:0000269|PubMed:21045126, ECO:0000269|PubMed:21277849, ECO:0000269|PubMed:23028315, ECO:0000269|PubMed:23382217, ECO:0000269|PubMed:24695226, ECO:0000269|PubMed:25238095, ECO:0000269|PubMed:25310967, ECO:0000269|PubMed:27845049, ECO:0000269|PubMed:29155995, ECO:0000269|PubMed:29305823, ECO:0000269|PubMed:29784761, ECO:0000269|PubMed:30262916}.

Molecular Weight: 62.3 kDa

UniProt: Q91W98

AlphaFold: Q91W98