DCP1A Protein (AA 1-602) (His tag)

Catalog No. ABIN7560100

This Recombinant DCP1A protein is produced in HEK-293 Cells.

Quick Overview for DCP1A Protein (AA 1-602) (His tag) (ABIN7560100)

Target: DCP1A (DCP1 Decapping Enzyme Homolog A (S. Cerevisiae) (DCP1A))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-602

Purification tag / Conjugate: This DCP1A protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Dcp1a Protein expressed in mammalian cells.

Sequence: MALSCSTVRP RRRGSALRSK MELLSRAEQE MSLAALKQHD PYITSIADLT GQVALYTFCP KANQWEKTDI EGTLFVYRRS ASPYHGFTIV NRLNMHNLVE PVNKDLEFQL HEPFLLYRNA SLSIYSIWFY DKNDCHRIAK LMADVVEEET RRSQQAARDK QSPSQANGCS DQRPIDILEM LSRAKDEYER NQMGGSNISS PGLQPSTQLS NLGSTETLEE TPSGSQDKSA PSGHKHLTVE ELFGTSLPKE QPTAMGLESE DTDKLLGDAS QKEPSSFLPF PFEQSGGAPQ SENLGIHSAA HHTVQPEVST PVLITPASIA QSGDKHPPSY TLPLSPVLSP TLPAEAPTTQ VPHLPRNSTM IQAVKTTPRQ KSPLLNQPVP ELSHSSLVAS QSPFRAPVSL ANPAGTALPS VDLLQKLRLT PQHDQIQAQP LGKGTMAPSF SSAAGQLATP ESFIEPSSKT AAARAAVSAS LSNMVLAPTL QSMQQNQDPE VFSQPKVLPS AIPIAGSPLV PATTTAVSSV LLSPSVFQQT VPRAADLERK ASSPSPLTVG TAESQRKPSI ILSKSQLQDT LIHLIKNDSS FLSTLHAVYL QVLTKNKDNH NL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: DCP1A (DCP1 Decapping Enzyme Homolog A (S. Cerevisiae) (DCP1A))

Alternative Name: Dcp1a

Background: MRNA-decapping enzyme 1A (EC 3.6.1.62) (MAD homolog 4-interacting transcription coactivator 1) (Smad4-interacting transcriptional co-activator) (Transcription factor SMIF),FUNCTION: Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1 (By similarity). Essential for embryonic development (PubMed:11836524). {ECO:0000250|UniProtKB:Q9NPI6, ECO:0000269|PubMed:11836524}.

Molecular Weight: 65.2 kDa

UniProt: Q91YD3

AlphaFold: Q91YD3