DDX27 Protein (AA 1-760) (His tag)

Catalog No. ABIN7560199

Product Details

Target: DDX27 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 27 (DDX27))

Protein Type: Recombinant

Protein Characteristics: AA 1-760

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This DDX27 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Ddx27 Protein expressed in mammalian cells.

Sequence: MLAELGFIRT IGENDEVPVE PESDSGDEEE EGPIVLGRKQ KALQKNRSAD FNPDFVFTEK EGMYDGSWAL ADVMSQLKKK RAATTLDEKI EKVRKRRKAE DKEAKSGKVE EKEGQADSDL KGQENPGEDE AGSKDEDSET DYSSEDEEIL TKADTLKVKE KKKKKKGQAA GGFFEDASEY DKSLSFQDMN LSRPLLKAIT AMGFKQPTPI QKACIPVGLL GKDICACAAT GTGKTAAFAL PVLERLIYKP RQAAVTRVLV LVPTRELGIQ VHSVTKQLAQ FCSITTCLAV GGLDVKSQEA ALRAAPDILI ATPGRLIDHL HNCPSFHLSS IEVLILDEAD RMLDEYFEEQ MKEIIRMCSH HRQTMLFSAT MTDEVKDLAS VSLKNPVRIF VNSNTDVAPF LRQEFIRIRP NREGDREAIV AALLMRTFTD HVMLFTQTKK QAHRMHILLG LLGLQVGELH GNLSQTQRLE ALRRFKDEQI DILVATDVAA RGLDIEGVKT VINFTMPNTV KHYVHRVGRT ARAGRAGRSV SLVGEEERKM LKEIVKAAKA PVKARILPQD VILKFRDKIE KLEKDVYAVL QLEAEEKEMQ QSEAQIDTAQ RLLAKGKETA DQEPERSWFQ TKEERKKEKI AKALQEFDLA LRGKKKRKKF MKDAKKKGEM TAEERSQFEI LKAQMFAERL AKRNRRTKRA RAMPEDEPTG PAKKQKQQQK SVFDEELTNT SKKALKQYRA GPSFEERKQS GLPRQRRGNF KSKSRYKRKK Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: DDX27 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 27 (DDX27))

Alternative Name: Ddx27 (DDX27 Products)

Background: Probable ATP-dependent RNA helicase DDX27 (EC 3.6.4.13) (DEAD box protein 27),FUNCTION: Probable ATP-dependent RNA helicase. Component of the nucleolar ribosomal RNA (rRNA) processing machinery that regulates 3' end formation of ribosomal 47S rRNA. {ECO:0000250|UniProtKB:Q96GQ7}.

Molecular Weight: 85.9 kDa

UniProt: Q921N6

AlphaFold: Q921N6