PRKX Protein (AA 1-355) (His tag)

Catalog No. ABIN7560237

Recombinant PRKX protein expressed in HEK-293 Cells.

Quick Overview for PRKX Protein (AA 1-355) (His tag) (ABIN7560237)

Target: PRKX (Protein Kinase, X-Linked (PRKX))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-355

Purification tag / Conjugate: This PRKX protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Prkx Protein expressed in mammalian cells.

Sequence: MEPPAGAAAT VKDPDHDPVK TKVSAPAADP KPRTSSQKAG HSLQDWDTIA TVGTGTFGRV NLVKEKTGRQ YCALKIMSIP DVIRLKQEQH VQNEKAVLKE INHPFLIKLL WTGHDNRFLY MLMEFVPGGE LFTYLRNRGR FSSVASVFYA TEIVCAIEYL HSKEIVYRDL KPENILLDRE GHIKLTDFGF AKKLVDRTWT LCGTPEYLAP EVIQSKGHGR AVDWWALGIL IFEMLSGFPP FFDDNPFGIY QKILACKIDF PRQLDFTSKD LIKKLLVVDR TRRLGNMKNG AEDIKRHRWF RGVEWESVPQ RKLKPPIVPK LSGDGDISNF ETYPESELDK TPSVSDKDLE TFKNF Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: PRKX (Protein Kinase, X-Linked (PRKX))

Alternative Name: Prkx

Background: CAMP-dependent protein kinase catalytic subunit PRKX (PrKX) (Protein kinase X) (Protein kinase X-linked) (Serine/threonine-protein kinase PRKX) (EC 2.7.11.1) (PKA-related protein kinase),FUNCTION: Serine/threonine protein kinase regulated by and mediating cAMP signaling in cells. Acts through phosphorylation of downstream targets that may include CREB, SMAD6 and PKD1 and has multiple functions in cellular differentiation and epithelial morphogenesis. Regulates myeloid cell differentiation through SMAD6 phosphorylation. Involved in nephrogenesis by stimulating renal epithelial cell migration and tubulogenesis. Also involved in angiogenesis through stimulation of endothelial cell proliferation, migration and vascular-like structure formation. {ECO:0000269|PubMed:16236808, ECO:0000269|PubMed:19367327, ECO:0000269|PubMed:21684272}.

Molecular Weight: 40.5 kDa

UniProt: Q922R0

AlphaFold: Q922R0

Pathways: Thyroid Hormone Synthesis