PNO1 Protein (AA 1-248) (His tag)

Catalog No. ABIN7560476

This Recombinant PNO1 protein is expressed in HEK-293 Cells.

Quick Overview for PNO1 Protein (AA 1-248) (His tag) (ABIN7560476)

Target: PNO1 (Partner of NOB1 Homolog (PNO1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-248

Purification tag / Conjugate: This PNO1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Pno1 Protein expressed in mammalian cells.

Sequence: METQSTGTED GFTPVTHRGG RRAKKRQAEQ SSAAGQDGEA GRMDTEEARP AKRPVFPPLS GDQLLTGKEE TRKIPVPGNR YTPLKENWMK IFTPIVEHLG LQIRFNLKSR NVEIRTCKDT KDVSALTKAA DFVKAFVLGF QVEDALALIR LDDLFLESFE ITDVKPLKGD HLSRAIGRIA GKGGKTKFTI ENVTRTRIVL ADVHVHILGS FQNIKMARTA ICNLILGNPP SKVYGNIRAV ASRSADRF Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: PNO1 (Partner of NOB1 Homolog (PNO1))

Alternative Name: Pno1

Background: RNA-binding protein PNO1,FUNCTION: Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Positively regulates dimethylation of two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA. {ECO:0000250|UniProtKB:Q9NRX1}.

Molecular Weight: 27.5 kDa

UniProt: Q9CPS7

AlphaFold: Q9CPS7