ATPBD4 Protein (AA 1-267) (His tag)
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- Target See all ATPBD4 Proteins
- ATPBD4 (ATP Binding Domain 4 (ATPBD4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-267
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ATPBD4 protein is labelled with His tag.
- Purpose
- Predefined custom protein recombinant Dph6 Protein expressed in mammalian cells.
- Sequence
- MRVAALISGG KDSCYNMMQC IAEGHQIVAL ANLRPDENQV ESDELDSYMY QTVGHHAIDL YAEAMALPLY RRAIRGRSLE TGRVYTQCEG DEVEDLYELL KLVKEKEEIE GVSVGAILSD YQRGRVENVC KRLNLQPLAY LWQRNQEDLL REMIASNIKA IIIKVAALGL DPDKHLGKTL VEMEPYLLEL SKKYGVHVCG EGGEYETFTL DCPLFKKKIV VDSSEAVMHS ADAFAPVAYL RLSRLHLEEK VSSVPADDET ANSIHSS Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
- Top Product
- Discover our top product ATPBD4 Protein
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ATP Binding Domain 4 (ATPBD4) (AA 1-267) protein (His tag)
custom-made ATPBD4 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
ATP Binding Domain 4 (ATPBD4) (AA 1-267) protein (GST tag)ATPBD4 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
ATP Binding Domain 4 (ATPBD4) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)ATPBD4 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- ATPBD4 (ATP Binding Domain 4 (ATPBD4))
- Alternative Name
- Dph6 (ATPBD4 Products)
- Background
- Diphthine--ammonia ligase (EC 6.3.1.14) (ATP-binding domain-containing protein 4) (Diphthamide synthase) (Diphthamide synthetase) (Protein DPH6 homolog),FUNCTION: Amidase that catalyzes the last step of diphthamide biosynthesis using ammonium and ATP. Diphthamide biosynthesis consists in the conversion of an L-histidine residue in the translation elongation factor 2 (EEF2) to diphthamide (By similarity). {ECO:0000250}.
- Molecular Weight
- 29.9 kDa
- UniProt
- Q9CQ28
- AlphaFold
- Q9CQ28
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