TRIM13 Protein (AA 1-407) (His tag)

Catalog No. ABIN7560750

Recombinant TRIM13 protein expressed in HEK-293 Cells.

Quick Overview for TRIM13 Protein (AA 1-407) (His tag) (ABIN7560750)

Target: TRIM13 (Tripartite Motif Containing 13 (TRIM13))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-407

Purification tag / Conjugate: This TRIM13 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Trim13 Protein expressed in mammalian cells.

Sequence: MELLEEDLTC PICCSLFDDP RVLPCSHNFC KKCLEGLLEG NVRNSLWRPS PFKCPTCRKE TSATGVNSLQ VNYSLKGIVE KYNKIKISPK MPVCKGHLGQ PLNIFCVTDM QLICGICATR GEHTKHVFSS IEDAYAREKN AFESLFQSFE TWRRGDALSR LDTLETNKRK ALQLLTKDSD KVKEFFEKLQ HTLDQKKNEI LSDFETMKLA VMQTYDPEIN KINTILQEQR MAFNIAEAFK DVSEPIIFLQ QMQEFREKIK VIKETPLPHS NLPTSPLMKN FDTSQWGDIK LVDVDKLSLP QDTGVFTSKI PWYPYLLLMM VVLLGLLIFF GPTVFLEWSP LDELATWKDY LSSFNSYLTK SADFIEQSVF YWEQMTDGFF IFGERVKNVS LVALNNVAEF ICKYKLL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TRIM13 (Tripartite Motif Containing 13 (TRIM13))

Alternative Name: Trim13

Background: E3 ubiquitin-protein ligase TRIM13 (EC 2.3.2.27) (Putative tumor suppressor RFP2) (RING-type E3 ubiquitin transferase TRIM13) (Ret finger protein 2) (Tripartite motif-containing protein 13),FUNCTION: Endoplasmic reticulum (ER) membrane anchored E3 ligase involved in the retrotranslocation and turnover of membrane and secretory proteins from the ER through a set of processes named ER-associated degradation (ERAD). This process acts on misfolded proteins as well as in the regulated degradation of correctly folded proteins. Enhances ionizing radiation-induced p53/TP53 stability and apoptosis via ubiquitinating MDM2 and AKT1 and decreasing AKT1 kinase activity through MDM2 and AKT1 proteasomal degradation. Regulates ER stress-induced autophagy, and may act as a tumor suppressor. Also plays a role in innate immune response by stimulating NF-kappa-B activity in the TLR2 signaling pathway. Ubiquitinates TRAF6 via the 'Lys-29'-linked polyubiquitination chain resulting in NF-kappa-B activation. Participates as well in T-cell receptor-mediated NF-kappa-B activation. In the presence of TNF, modulates the IKK complex by regulating IKBKG/NEMO ubiquitination leading to the repression of NF-kappa-B. {ECO:0000250|UniProtKB:O60858}.

Molecular Weight: 47.0 kDa

UniProt: Q9CYB0

AlphaFold: Q9CYB0