RABL4 Protein (AA 1-186) (His tag)

Catalog No. ABIN7560849

Recombinant RABL4 protein expressed in HEK-293 Cells.

Quick Overview for RABL4 Protein (AA 1-186) (His tag) (ABIN7560849)

Target: RABL4 (IFT27) (Intraflagellar Transport 27 Homolog (IFT27))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-186

Purification tag / Conjugate: This RABL4 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Ift27 Protein expressed in mammalian cells.

Sequence: MVKLAAKCIL AGDPAVGKTA LVQMFRSDGT HFQKNYTLTT GVDLVVKTVP VLDTNDSVEL FIFDSAGKEL FSEMLDKLWE NPNVLCLVYD VTNEQSFISC TKWLEKVRSQ TSGISLPGVL VGTKTDLAGR QTVDSAQAQV WALSQGLEFF ETSVKEMDNY EAPFHCLAKQ FYQLYREKVD IFHTLV Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: RABL4 (IFT27) (Intraflagellar Transport 27 Homolog (IFT27))

Alternative Name: Ift27

Background: Intraflagellar transport protein 27 homolog (Putative GTP-binding protein RAY-like) (Rab-like protein 4),FUNCTION: Small GTPase-like component of the intraflagellar transport (IFT) complex B that promotes the exit of the BBSome complex from cilia via its interaction with ARL6 (PubMed:25446516). Not involved in entry of the BBSome complex into cilium. Prevents aggregation of GTP-free ARL6. Required for hedgehog signaling (PubMed:25446516). Forms a subcomplex within the IFT complex B with IFT25 (By similarity). Its role in intraflagellar transport is mainly seen in tissues rich in ciliated cells such as kidney and testis. Essential for male fertility, spermiogenesis and sperm flagella formation (PubMed:28964737). Plays a role in the early development of the kidney (PubMed:29626631). May be involved in the regulation of ureteric bud initiation (PubMed:29626631). {ECO:0000250|UniProtKB:A8HN58, ECO:0000269|PubMed:25446516, ECO:0000269|PubMed:28964737, ECO:0000269|PubMed:29626631}.

Molecular Weight: 20.8 kDa

UniProt: Q9D0P8

AlphaFold: Q9D0P8

Pathways: Hedgehog Signaling