AP1S2 Protein (AA 1-160) (His tag)
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- Target See all AP1S2 Proteins
- AP1S2 (Adaptor-Related Protein Complex 1, sigma 2 Subunit (AP1S2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-160
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This AP1S2 protein is labelled with His tag.
- Purpose
- Made-to-order recombinant Ap1s2 Protein expressed in mammalian cells.
- Sequence
- MQFMLLFSRQ GKLRLQKWYV PLSDKEKKKI TRELVQTVLA RKPKMCSFLE WRDLKIVYKR YASLYFCCAI EDQDNELITL EIIHRYVELL DKYFGSVCEL DIIFNFEKAY FILDEFLLGG EVQETSKKNV LKAIEQADLL QEDAKEAETP RSVLEEIGLT Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Adaptor-Related Protein Complex 1, sigma 2 Subunit (AP1S2) (AA 1-157) protein (His tag)
custom-made AP1S2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Adaptor-Related Protein Complex 1, sigma 2 Subunit (AP1S2) (AA 1-157) protein (His tag)AP1S2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Adaptor-Related Protein Complex 1, sigma 2 Subunit (AP1S2) (AA 1-157) protein (GST tag)AP1S2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- AP1S2 (Adaptor-Related Protein Complex 1, sigma 2 Subunit (AP1S2))
- Alternative Name
- Ap1s2 (AP1S2 Products)
- Background
- AP-1 complex subunit sigma-2 (Adaptor protein complex AP-1 subunit sigma-1B) (Adaptor-related protein complex 1 subunit sigma-1B) (Clathrin assembly protein complex 1 sigma-1B small chain) (Golgi adaptor HA1/AP1 adaptin sigma-1B subunit) (Sigma 1B subunit of AP-1 clathrin) (Sigma-adaptin 1B) (Sigma1B-adaptin),FUNCTION: Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules (By similarity). {ECO:0000250}.
- Molecular Weight
- 18.9 kDa
- UniProt
- Q9DB50
- AlphaFold
- Q9DB50
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