PANX1 Protein (AA 1-426) (His tag)

Catalog No. ABIN7561551

Product Details

Target: PANX1 (Pannexin 1 (PANX1))

Protein Type: Recombinant

Protein Characteristics: AA 1-426

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This PANX1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Panx1 Protein expressed in mammalian cells.

Sequence: MAIAHLATEY VFSDFLLKEP TEPKFKGLRL ELAVDKMVTC IAVGLPLLLI SLAFAQEISI GTQISCFSPS SFSWRQAAFV DSYCWAAVQQ KSSLQSESGN LPLWLHKFFP YILLLFAILL YLPALFWRFS AAPHLCSDLK FIMEELDKVY NRAIKAAKSA RDLDLRDGPG PPGVTENVGQ SLWEISESHF KYPIVEQYLK TKKNSSHLIM KYISCRLVTF VVILLACIYL SYYFSLSSLS DEFLCSIKSG VLKNDSTIPD RFQCKLIAVG IFQLLSLINL IVYALLIPVV VYTFFIPFRQ KTDILKVYEI LPTFDVLHFK SEGYNDLSLY NLFLEENISE LKSYKCLKVL ENIKSNGQGI DPMLLLTNLG MIKMDIIDGK IPTSLQTKGE DQGSQRVEFK DLDLSSEAAA NNGEKNSRQR LLNPSC Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: PANX1 (Pannexin 1 (PANX1))

Alternative Name: Panx1 (PANX1 Products)

Background: Pannexin-1 [Cleaved into: Caspase-activated pannexin-1 (Caspase-activated PANX1)],FUNCTION: Ion channel involved in a variety of physiological functions such as blood pressure regulation, apoptotic cell clearance and oogenesis (PubMed:30814251). Forms anion-selective channels with relatively low conductance and an order of permeabilities: nitrate>iodide>chlroride>>aspartate=glutamate=gluconate (PubMed:22311122). Can release ATP upon activation through phosphorylation or cleavage at C-terminus (PubMed:32238926). May play a role as a Ca(2+)-leak channel to regulate ER Ca(2+) homeostasis (By similarity). {ECO:0000250|UniProtKB:Q96RD7, ECO:0000269|PubMed:22311122, ECO:0000269|PubMed:30814251, ECO:0000269|PubMed:32238926}., FUNCTION: [Caspase-activated pannexin-1]: During apoptosis and after cleavage by caspases of the C-terminal tail, acts as a plasma membrane channel which mediates the regulated release of find-me signals, such as nucleotides ATP and UTP, and selective plasme membrane permeability. {ECO:0000269|PubMed:32238926}.

Molecular Weight: 48.2 kDa

UniProt: Q9JIP4

AlphaFold: Q9JIP4