P2RX4 Protein (AA 1-388) (His tag)

Catalog No. ABIN7561614

Recombinant P2RX4 protein expressed in HEK-293 Cells.

Quick Overview for P2RX4 Protein (AA 1-388) (His tag) (ABIN7561614)

Target: P2RX4 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 4 (P2RX4))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-388

Purification tag / Conjugate: This P2RX4 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant P2rx4 Protein expressed in mammalian cells.

Sequence: MAGCCSVLGS FLFEYDTPRI VLIRSRKVGL MNRVVQLLIL AYVIGWVFVW EKGYQETDSV VSSVTTKAKG VAVTNTSQLG FRIWDVADYV VPAQEENSLF IMTNMIVTVN QTQGTCPEIP DKTSICDSDA NCTLGSSDTH SSGIGTGRCV PFNASVKTCE VAAWCPVEND AGVPTPAFLK AAENFTLLVK NNIWYPKFNF SKRNILPNIT TSYLKSCIYN ARTDPFCPIF RLGQIVADAG HSFQEMAVEG GIMGIQIKWD CNLDRAASHC LPRYSFRRLD TRDLEHNVSP GYNFRFAKYY RDLAGNEQRT LTKAYGIRFD IIVFGKAGKF DIIPTMINVG SGLALLGVAT VLCDVIVLYC MKKRYYYRDK KYKYVEDYEQ GLSGEMNQ Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: P2RX4 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 4 (P2RX4))

Alternative Name: P2rx4

Background: P2X purinoceptor 4 (P2X4) (ATP receptor) (Purinergic receptor),FUNCTION: ATP-gated nonselective transmembrane cation channel permeable to potassium, sodium and calcium. Activated by extracellularly released ATP, it plays multiple role in immunity and central nervous system physiology (PubMed:26456657, PubMed:35119925). Plays a key role in initial steps of T-cell activation and Ca(2+) microdomain formation (PubMed:35119925). Participates also in basal T-cell activity without TCR/CD3 stimulation (PubMed:35119925). Promotes the differentiation and activation of Th17 cells via expression of retinoic acid-related orphan receptor C/RORC (By similarity). Upon activation, drives microglia motility via the PI3K/Akt pathway (PubMed:17299767). Could also function as an ATP-gated cation channel of lysosomal membranes (PubMed:27477609). {ECO:0000250|UniProtKB:Q99571, ECO:0000269|PubMed:17299767, ECO:0000269|PubMed:26456657, ECO:0000269|PubMed:27477609, ECO:0000269|PubMed:35119925}.

Molecular Weight: 43.4 kDa

UniProt: Q9JJX6

AlphaFold: Q9JJX6

Pathways: Synaptic Membrane