SRF Protein (AA 1-504) (His tag)

Catalog No. ABIN7561695

Recombinant SRF protein expressed in HEK-293 Cells.

Quick Overview for SRF Protein (AA 1-504) (His tag) (ABIN7561695)

Target: SRF (Serum Response Factor (SRF))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-504

Purification tag / Conjugate: This SRF protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Srf Protein expressed in mammalian cells.

Sequence: MLPSQAGAAA ALGRGSALGG NLNRTPTGRP GGGGGTRGAN GGRVPGNGAG LGQSRLEREA AAAAAPTAGA LYSGSEGDSE SGEEEELGAE RRGLKRSLSE MELGVVVGGP EAAAAAAGGY GPVSGAVSGA KPGKKTRGRV KIKMEFIDNK LRRYTTFSKR KTGIMKKAYE LSTLTGTQVL LLVASETGHV YTFATRKLQP MITSETGKAL IQTCLNSPDS PPRSDPTTDQ RMSATGFEEP DLTYQVSESD SSGETKDTLK PAFTVTNLPG TTSTIQTAPS TSTTMQVSSG PSFPITNYLA PVSASVSPSA VSSANGTVLK STGSGPVSSG GLMQLPTSFT LMPGGAVAQQ VPVQAIHVHQ APQQASPSRD SSTDLTQTSS SGTVTLPATI MTSSVPTTVG GHMMYPSPHA VMYAPTSGLA DGSLTVLNAF SQAPSTMQVS HSQVQEPGGV PQVFLTAPSG TVQIPVSAVQ LHQMAVIGQQ AGSSSNLTEL QVVNLDATHS TKSE Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SRF (Serum Response Factor (SRF))

Alternative Name: Srf

Background: Serum response factor (SRF),FUNCTION: SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS) (PubMed:24732378). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration (PubMed:12732141, PubMed:19350017, PubMed:24732378). The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics (PubMed:24732378). Required for cardiac differentiation and maturation (PubMed:15169892). {ECO:0000269|PubMed:12732141, ECO:0000269|PubMed:15169892, ECO:0000269|PubMed:19350017, ECO:0000269|PubMed:24732378}.

Molecular Weight: 51.2 kDa

UniProt: Q9JM73

AlphaFold: Q9JM73