CLEC4A Protein (AA 1-238) (His tag)

Catalog No. ABIN7561809

Recombinant CLEC4A protein expressed in HEK-293 Cells.

Quick Overview for CLEC4A Protein (AA 1-238) (His tag) (ABIN7561809)

Target: CLEC4A (C-Type Lectin Domain Family 4, Member A (CLEC4A))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-238

Purification tag / Conjugate: This CLEC4A protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Clec4a Protein expressed in mammalian cells.

Sequence: MASEITYAEV KFKNESNSLH TYSESPAAPR EKPIRDLRKP GSPSLLLTSL MLLLLLLAIT FLVAFIIYFQ KYSQLLEEKK AAKNIMHNEL NCTKSVSPME DKVWSCCPKD WRLFGSHCYL VPTVSSSASW NKSEENCSRM GAHLVVIQSQ EEQDFITGIL DTHAAYFIGL WDTGHRQWQW VDQTPYEESI TFWHNGEPSS GNEKCATIIY RWKTGWGWND ISCSLKQKSV CQMKKINL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: CLEC4A (C-Type Lectin Domain Family 4, Member A (CLEC4A))

Alternative Name: Clec4a

Background: C-type lectin domain family 4 member A (C-type lectin superfamily member 6) (Dendritic cell immunoreceptor) (CD antigen CD367),FUNCTION: May be involved in regulating immune reactivity. May play a role in modulating dendritic cells (DC) differentiation and/or maturation (By similarity). May be involved in the inhibition of B-cell-receptor-mediated calcium mobilization and protein tyrosine phosphorylation. {ECO:0000250, ECO:0000269|PubMed:11841542}., FUNCTION: C-type lectin receptor that binds carbohydrates mannose and fucose but also weakly interacts with N-acetylglucosamine (GlcNAc) in a Ca(2+)-dependent manner. Involved in regulating immune reactivity. Once triggered by antigen, it is internalized by clathrin-dependent endocytosis and delivers its antigenic cargo into the antigen presentation pathway resulting in cross-priming of CD8(+) T cells. This cross-presentation and cross-priming are enhanced by TLR7 and TLR8 agonists with increased expansion of the CD8(+) T cells, high production of IFNG and TNF with reduced levels of IL4, IL5 and IL13. In plasmacytoid dendritic cells, inhibits TLR9-mediated IFNA and TNF production (By similarity). May be involved via its ITIM motif (immunoreceptor tyrosine-based inhibitory motifs) in the inhibition of B-cell-receptor-mediated calcium mobilization and protein tyrosine phosphorylation (PubMed:11841542). {ECO:0000250|UniProtKB:Q9UMR7, ECO:0000269|PubMed:11841542}.

Molecular Weight: 27.3 kDa

UniProt: Q9QZ15

AlphaFold: Q9QZ15