DHX8 Protein (AA 1-1244) (His tag)

Catalog No. ABIN7561863

Product Details

Target: DHX8 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 8 (DHX8))

Protein Type: Recombinant

Protein Characteristics: AA 1-1244

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This DHX8 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Dhx8 Protein expressed in mammalian cells.

Sequence: MAVAVAAAGV LMGSEPGPAE ELAKLEYLSL VSKVCTELDN HLGINDKDLA EFVISLAEKN TTFDTFKASL VKNGAEFTDS LISNLLRLIQ TMRPPAKPST SKDPVVKPKT EKEKLRELFP VLCQPDNPSA RTMLDEEDVK VAVDVLKELE ALMPSAAGQE KQRDPEHRDR TKKKKRSRSR DRDRDRDRDR DRDRDRDRDR DKDRERDRDR ERDRERDRER DHKRRHRSRS RSHSRTRERT KGKSRYRSRS RSQSPFKDRK DREKYGERNL DRWRDKHVDR PPPEEPAIGD IYNGKVTSIM QFGCFVQLEG LRKRWEGLVH ISELRREGRV ANVADVVSKG QRVKVKVLSF TGTKTSLSMK DVDQETGEDL NPNRRRNLVG ETNEETSMRN PDRPTHLSLV SAPEVEDDSL ERKRLTRISD PEKWEIKQMI AANVLSKEEF PDFDEETGIL PKVDDEEDED LEIELVEEEP PFLRGHTKQS MDMSPIKIVK NPDGSLSQAA MMQSALAKER RELKQAQREA EMDSIPMGLN KHWVDPLPDA EGRQIAANMR GIGMMPNDIP EWKKHAFGGN KASYGKKTQM SILEQRESLP IYKLKEQLVQ AVHDNQILIV IGETGSGKTT QITQYLAEAG YTSRGKIGCT QPRRVAAMSV AKRVSEEFGC CLGQEVGYTI RFEDCTSPET VIKYMTDGML LRECLIDPDL TQYAIIMLDE AHERTIHTDV LFGLLKKTVQ KRQDMKLIVT SATLDAVKFS QYFYEAPIFT IPGRTYPVEI LYTKEPETDY LDASLITVMQ IHLTEPPGDI LVFLTGQEEI DTACEILYER MKSLGPDVPE LIILPVYSAL PSEMQTRIFD PAPPGSRKVV IATNIAETSL TIDGIYYVVD PGFVKQKVYN SKTGIDQLVV TPISQAQAKQ RAGRAGRTGP GKCYRLYTER AYRDEMLTTN VPEIQRTNLA STVLSLKAMG INDLLSFDFM DAPPMETLIT AMEQLYTLGA LDDEGLLTRL GRRMAEFPLE PMLCKMLIMS VHLGCSEEML TIVSMLSVQN VFYRPKDKQA LADQKKAKFH QTEGDHLTLL AVYNSWKNNK FSNPWCYENF IQARSLRRAQ DIRKQMLGIM DRHKLDVVSC GKSTVRVQKA ICSGFFRNAA KKDPQEGYRT LIDQQVVYIH PSSALFNRQP EWVVYHELVL TTKEYMREVT TIDPRWLVEF APAFFKVSDP TKLSKQKKQQ RLEPLYNRYE EPNAWRISRA FRRR Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: DHX8 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 8 (DHX8))

Alternative Name: Dhx8 (DHX8 Products)

Background: ATP-dependent RNA helicase DHX8 (EC 3.6.4.13) (DEAH box protein 8),FUNCTION: Involved in pre-mRNA splicing as component of the spliceosome. Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome. {ECO:0000250|UniProtKB:Q14562}.

Molecular Weight: 142.6 kDa

UniProt: A2A4P0

AlphaFold: A2A4P0