NDOR1 Protein (AA 1-598) (His tag)

Catalog No. ABIN7561914

Recombinant NDOR1 protein expressed in HEK-293 Cells.

Quick Overview for NDOR1 Protein (AA 1-598) (His tag) (ABIN7561914)

Target: NDOR1 (NADPH Dependent Diflavin Oxidoreductase 1 (NDOR1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-598

Purification tag / Conjugate: This NDOR1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Ndor1 Protein expressed in mammalian cells.

Sequence: MQVPQLLVLF GSQTGTAQDE AERLGREARR RRLGCRVQAL DSYSVANLIR EPLVIFVCAT TGQGDPPDNM KNFWRFIFRK SLPSSSLCQM DFAVLGLGDS SYAKFNFVAK KLHRRLLQLG GSALLPPCLG DDQHELGPDA AIDPWVGDLW EKIMVMYPVP LDIPEIPHGV PLPSKFIFQF LQEVPSIGAE ELNIASSAPQ TPPSELQPFL APVITNQRVT GPQHFQDVRL IEFDITDSNI SFAAGDVVFI LPSNSEAHTQ QFCQVLCLDP NQFFTLKPRE PGVPDPPGLP QPCTVWNLVS QYLDIASVPR RSFFELLACL SQHALEREKL LELSSARGQE ELWEYCSRPR RTILEVLCDF PHTAGAIPPD YLLDLIPRIR PRAFSIASSL LAHPRRLQIL VAVVKYQTRL KEPRHGLCSS WLASLNPGQA GPVRVPLWVR PGSLVFPKTP DTPIIMVGAG TGVAPFRAAI QERVAHGQTG NFLFFGCRQR DQDFYWQTEW QKLEQKGWLT LVTAFSREQE QKVYVQHRLR ELGPLVWELL DGQGAYFYLA GNAKYLPTDV SEALMSIFQE EGRLSTADAS AYLARLQQTL RFQTETWA Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: NDOR1 (NADPH Dependent Diflavin Oxidoreductase 1 (NDOR1))

Alternative Name: Ndor1

Background: NADPH-dependent diflavin oxidoreductase 1 (EC 1.18.1.-) (NADPH-dependent FMN and FAD-containing oxidoreductase),FUNCTION: NADPH-dependent reductase which is a central component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Transfers electrons from NADPH via its FAD and FMN prosthetic groups to the [2Fe-2S] cluster of CIAPIN1, another key component of the CIA machinery. In turn, this reduced cluster provides electrons for assembly of cytosolic iron-sulfur cluster proteins. It can also reduce the [2Fe-2S] cluster of CISD1 and activate this protein implicated in Fe/S cluster repair (By similarity). In vitro can fully activate methionine synthase/MTR in the presence of soluble cytochrome b5/CYB5A (By similarity). {ECO:0000250|UniProtKB:Q9UHB4, ECO:0000255|HAMAP-Rule:MF_03178}.

Molecular Weight: 67.2 kDa

UniProt: A2AI05

AlphaFold: A2AI05