MAP3K15 Protein (AA 1-1331) (His tag)

Catalog No. ABIN7561947

Recombinant MAP3K15 protein expressed in HEK-293 Cells.

Quick Overview for MAP3K15 Protein (AA 1-1331) (His tag) (ABIN7561947)

Target: MAP3K15 (Mitogen-Activated Protein Kinase Kinase Kinase 15 (MAP3K15))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1331

Purification tag / Conjugate: This MAP3K15 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Map3k15 Protein expressed in mammalian cells.

Sequence: MEGGGGSGGG GGPVPAEAPE EAGEPPQGRL PPGPEGAAGL AEPESTGDAA GGEAEGGRGP RRALRAVYVR SESSQGAAAG GGPEAGALKC LLRACEAEGA HLTSVPFGEL DFGETAVLDA FYDADVAIVD MSDISRQPSL FYHLGVRESF DMANNVILYY DTDADTALSL KDMVTQKNTA SSGNYYFIPY TVTPCADYFC CESDAQRRAS EYMQPNWDTI LGPLCMPLVD RFTSLLKDIR VTSCAYYKET LLNDIRKARE KYQGDELAKE LTRIKFRMDN IEVLTSDIII NLLLSYRDIQ DYDAMVKLVE TLKMLPTCDL ADQHNIKFHY AFALNRRNST GDREKALQVM LQVLQSCDHP APDMFCLCGR IYKDIFLDSG CEEDASRDSA IEWYRKGFEL QSSLYSGINL AVLLIVSGQQ FETSMELRKI GVRLNSLLGR KGSLEKMNNY WDVGQFFTVS MLASDIGKAV QAAERLFKLK PPVWYLRSLV QNLLLIQRFK KPITEHSPRQ ERLNFWLDII FEATNEVTNG LRFPVLVIEP TKVYQPSYVS INNEAEERTV SLWHVSPTEM KQIHEWNFTA SSIKGISLSK FDERCCFLYV HDNSDDFQIY FSTEDQCNRF CSLVKEMLNN GVGSTVELEG EADGDTLEYE YDHDANGERV VLGKGSYGIV YAGRDLSNQV RIAIKEIPER DIRYSQPLHE EIALHKYLKH RNIVQYLGSV SENGYIKIFM EQVPGGSLSA LLRSKWGPMK EPTIKFYTKQ ILEGLKYLHE NQIVHRDIKG DNVLVNTYSG VVKISDFGTS KRLAGINPCT ETFTGTLQYM APEIIDQGPR GYGAPADIWS LGCTIIEMAT SRPPFHELGE PQAAMFKVGM FKIHPEIPEA LSAEARAFIL SCFEPDPQKR VTAADLLQEG FLRQVNKGKK NRIAFKPSEG VRSGTGTLAL PSSGELVGSS SSEHGSISPD SDAQPDAFFE KVQVPKHQLS HLLSVPDESP ALDDRSTALP PEERDPGLFL LRKDSERRAI LYRILWEEQN QVASNLQECV VQSSEELLLS VSHIKQIIGI LRDFIRSPEH RVMAATISKL KVDLDFDSSS INQIHLILFG FQDAVNRILR NHLIRPHWMF AMDNIIRRAV QAAVTILIPE LQAHFEPASE TEGVDKDTEV EGDYPLVDLL SQEVHVTPRG TRPGSVAIQE GQPHQQDPSL QLSKLRQETN RLWEHLVQKE REYQNLLRLI LDQKTQELYH LQLQYKSNGG TENPPPPDGL GTDRELIDWL QLQGVDANTI EKIVEEDYTL SDILNDITKE DLRCLRLRGG VLCRLWHAVS QHRRQMQESS Q Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: MAP3K15 (Mitogen-Activated Protein Kinase Kinase Kinase 15 (MAP3K15))

Alternative Name: Map3k15

Background: Mitogen-activated protein kinase kinase kinase 15 (EC 2.7.11.25) (MAPK/ERK kinase kinase 15) (MEK kinase 15) (MEKK 15),FUNCTION: Serine/threonine kinase which acts as a component of the MAP kinase signal transduction pathway. Once activated, acts as an upstream activator of the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases. May function in a signal transduction pathway that is activated by various cell stresses and leads to apoptosis. Involved in phosphorylation of WNK4 in response to osmotic stress or hypotonic low-chloride stimulation via the p38 MAPK signal transduction cascade. {ECO:0000250|UniProtKB:Q6ZN16}.

Molecular Weight: 149.3 kDa

UniProt: A2AQW0

AlphaFold: A2AQW0